摘要
目的:构建了一株直接利用木薯淀粉为原料生产异丁醇的重组菌株。方法:将地衣芽孢杆菌的淀粉酶基因克隆到生产异丁醇的大肠杆菌重组菌中,使大肠杆菌能够直接利用木薯淀粉为原料生产异丁醇。结果:木薯淀粉不需要预先加入淀粉酶进行糖化处理,就可以直接被作者研究所构建的重组菌株发酵生成异丁醇。结论:提供了一种直接发酵木薯淀粉生产异丁醇的方法。导入地衣芽孢杆菌的淀粉酶基因,能使只能利用葡萄糖作为发酵材料的异丁醇生产工程菌直接利用木薯淀粉为原料生产异丁醇。
Objective： To realize the isobutanol production in Escherichia coli with cassava starch, an engineering strains deficient in pflB, frdAB,fnr and fnrE and harboring double - plasmids, were constructed and investigated the changes in isobutanol of them in detail. Method：The amyl gene, encoding amylase from Bacillus licheniformis, was cloned and constructed the plasmid pse380 -amyl. And then this plasmid was transformed into the isobutanol producer engineering strain BW25113 ApflB Aft＇dAB Afnr AfnrE and obtained the recombinant stain gxas -AIKA,which can directly produce isobutanol using cassava starch as the raw material. Result：In the isobutanol fermentation with the recombinant strain gxas - AIKA, cassava starch was not needed to pre - add amylase for saccharification process, and could be directly fermented to produce isobutanoL Conclusion：This study will provide a method for directly fermenting cassava starch to produce isobutano]. Based on the engineering strain BW25113,which can only use glucose as carbon source to produce isobutanol, the am- ylase gene was successfully introduced and expressed in the engineering strain, making the use of cassava starch as raw materials to pro- duce isobutanol possible.
出处
《生物技术》
CAS
CSCD
北大核心
2014年第1期72-75,共4页
Biotechnology
基金
广西科学院基本科研业务项目("构建直接利用木薯淀粉发酵生产异丁醇的基因工程菌的研究"
11YJ24SW03
"环糊精水解酶水解大分子底物的分子结构基础和关键氨基酸研究"
12YJ25SW05)
广西科技攻关项目("高产率丁醇发酵菌株的构建及高浓度丁醇的发酵工艺优化"
10123007-3)资助
关键词
木薯淀粉
重组菌株
异丁醇
淀粉酶基因
Cassava starch
Recombinant strains
Isobutanol
Amylase gene
