摘要
目的:由希金斯炭疽菌侵染菜心、萝卜等十字花科蔬菜引起的炭疽病,不仅降低了蔬菜的产量,还影响着蔬菜的品质。RGS作为植物病原菌G蛋白信号转导过程中的重要调节因子,在众多生理生化过程中发挥着重要作用。对希金斯炭疽菌中典型RGS进行明确以及生物信息学分析,为深入开展该菌RGS功能研究打下坚实的理论基础。方法:基于酿酒酵母中4个典型RGS序列,利用Blastp以及关键词对希金斯炭疽菌蛋白质数据库进行比对、搜索,以及通过SMART保守结构域分析,获得典型的RGS,并对上述RGS进行二级结构、疏水性、信号肽以及亚细胞定位等生物信息学分析。结果:希金斯炭疽菌中的RGS在蛋白质二级结构中均含有较高比例的α螺旋结构,均不含有典型的信号肽序列,均为亲水性蛋白;其中3个定位在细胞核中,其他2个则分别定位于高尔基体、线粒体上。结论:希金斯炭疽菌中有5个典型的RGS,在二级结构特征、亲水性方面一致,但其亚细胞定位位置不同。
Objective：Colletotrichum higginsianum can infect flowering Chinese cabbage, carrot and other cruciferous vegetables, not only reduces the production of vegetables, but also affects the quality of vegetables. RGS as an important regulatory factor of plant pathogens G protein signal transduction pathway, play an important role in many physiological and biochemical processes. The study can provide strong theoretical foundation to further research the function of RGS in C. higginsianum. Method： Based on four typical RGS sequences in Saccha- romyces cerevisiae, to search RGS related protein sequence from the protein databases of Colletotrichum spp. with the Blastp as well as the use of keywords, and five typical RGS is identified by conserved domain analysis in the SMART online. Meanwhile, through bioinformatics analysis including the secondary structure of the amino acid sequence, hydrophobicity, signal peptide and sub- cellular localization. Result： There are higher proportions of helical secondary structure in every RGS protein, and which not contain the typical sig- nal peptide sequence,both hydrophihc protein,and three RGSs positioned in the nucleus, and the other is positioned in the Golgi, mito- chondria, respectively. Conclusion：There are five typical RGSs in C. higginsianum, their character of secondary structure and the hydro- philic aspects are same, but their sub -cellular localization are different.
出处
《生物技术》
CAS
CSCD
北大核心
2014年第1期36-41,共6页
Biotechnology
基金
云南省森林灾害预警与控制重点实验室开放基金项目("滇西地区核桃炭疽病菌的防治及多菌灵对其作用机制研究"
编号:ZK11A101
"滇西地区核桃炭疽病菌的鉴定及对多菌灵抗药性研究"
编号:ZK11SB01)
云南省重点学科森林保护学科研项目(编号:XKZ200905)资助
