摘要
目的测定不同类型角质形成细胞（KC）桥粒芯糖蛋白（DSG）1、DSG3及DSG1mRNA、DSG3mRNA表达。方法不同KC（HaCaT细胞、A431细胞及原代KC）培养后，直接免疫荧光观察细胞DSG1、DSG3的表达，定量聚合酶链反应（qPCR）测定DSG1mRNA、DSG3mRNA相对表达水平。结果DSG1与DSG3表达于三种角质形成细胞，荧光强度显示，A431细胞高于HaCaT细胞，HaCaT细胞高于原代KC。三种细胞均表达DSG1、DSG3mRNA，原代KCDSG1与DSG3mRNA的相对表达量分别为HaCaT细胞的291．7％及237．4％，差异有统计学意义（P〈0．01）；A431细胞DSG1mRNA为HaCaT细胞的0．1％、DSG3mRNA则为HaCaT细胞的18．8％，差异有统计学意义（P〈0．05）。结论三种KC均可用于对DSG1、DSG3的相关研究；相对而言，正常培养的A431细胞表面DSG1、DSG3表达较HaCaT细胞及原代KC高；原代KCDSG1、DSG3mRNA水平较HaCaT细胞及A431细胞高。
Objective To detect the mRNA and protein expressions of desmoglein 1 （DSG1） and DSG3 in different types of keratinocytes （KCs）. Methods Two keratinocyte cell lines HaCaT and A431, as well as primary keratinocytes from human abdomenal skin served as the object of this study. Direct immunofluorescenee assay was performed to observe and quantify the expressions of DSG1 and DSG3, and quantitative PCR （qPCR） to determine the mRNA expressions of DSG1 and DSG3, in these cells. Results Both DSG1 and DSG3 were expressed in all the three types of keratinocytes, and the fluorescence intensity of DSG1 and DSG3 in HaCaT ceils was higher than that in primary keratinocytes but lower than that in A431 cells. Similarly, all the keratinocytes expressed DSG1 and DSG3 mRNA, with the relative expression levels of DSG1 and DSG3 mRNA in primary keratinocytes being 291.7% and 237.4% of those in HaCaT cells respectively （both P 〈 0.01）, and those in A431 cells being 0.1% and 18.8% of those in HaCaT cells respectively （both P 〈 0.05）. Conclusions HaCaT cells, A431 cells and primary keratinocytes all can be used for the study of DSG1 and DSG3, of which, A431 cells show the strongest expressions of DSG1 and DSG3, and primary keratinocytes display the highest expressions of DSG1 and DSG3 mRNAs.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2014年第3期197-200,共4页
Chinese Journal of Dermatology
基金
国家自然科学基金（81071295）
