摘要
目的：黄芩苷是否通过抑制5-脂氧合酶（5-LOX）活性减轻大鼠脑缺血损伤诱导的炎症反应和脂质过氧化反应。方法：以成年雄性SD大鼠为研究对象，线栓法阻塞大鼠大脑中动脉（MCAO）建立脑缺血模型，检测缺血脑组织髓过氧化物酶（MPO）的活性和丙二醛（MDA）的含量，免疫组织化学检测5-LOX的表达水平，免疫印迹检测NF-κB的转录活性，ELISA法检测血清5-LOX代谢物白三烯B4（LTB4）和半胱氨酸白三烯（CysLT）的含量。结果：黄芩苷减轻炎症反应的浸润和脂质过氧化反应，降低5-LOX和NF-κB的表达，减少LTB4和CysLT的产生。结论：黄芩苷可减轻脑缺血炎症和脂质过氧化反应，可能是通过抑制5-LOX活性而起作用的。
Objective： Previous studies showed that baicalin possesses neuroprotective effects against cerebral ischemia. This study was designed to explore whether baicalin inhibits inflammatory reaction and lipid peroxidation via inhibiting the activation of 5-1ipoxygenase （LOX） in cerebral ischemia in rat. Methods： Adult Sprague-Dawley rats undergoing the permanent middle cerebral artery occlusion （pMCAO） were randomly divided into sham-operation, pMCAO and baicalin- treatment groups. Myeloperoxidase （MPO） activity in rat brain was measured as an index of neutrophil infiltration; MDA content in rat brain was measured as an index of lipid peroxidation; the expression of 5-LOX in rat brain was assessed by immunohistochemistry; the activity of NF-κB was detected by Western blotting; 5-LOX metabolites （LTB4 and CysLT） were determined by ELISA. Results： Baicalin reduced the enzymatic activity of MPO and the content of MDA, inhibited the expression of 5-LOX and the activity of NF-κB, and down-regulated the production of LTB4 and CysLT. Conclusion： Baicalin reduces inflammatory reaction and lipid peroxidation, which might inhibit the activation of 5-LOX in cerebral ischemia in rat.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2014年第1期51-53,91,共4页
Chinese Journal of Anatomy
基金
国家自然科学基金（81100987）,教育部博士点基金新教师类（20113518120005）,福建省自然科学基金（20lij05066）
