摘要
目的：探讨黄芩茎叶总黄酮（SSTF）对局灶性脑缺血再灌注（I／R）大鼠皮质Fas、FasL表达和超微结构的保护作用。方法：SD大鼠随机分为3组，假手术组、缺血再灌注组和SSTF组。SSTF组术前1周给予SSTF，其余2组给予等量PBS。各组大鼠分别灌胃给药1周后，采用线栓法制备局灶性脑缺血再灌注模型，缺血2h再灌注24h后观察大脑皮质病理形态学改变，检测Fas、FasL蛋白的表达以及神经元超微结构的改变。结果：SSTF可减轻损伤脑皮质的病理学改变。与I／R组比较，SSTF组皮质Fas、FasL阳性细胞明显减少；神经元较正常，核模较完整，线粒体损伤减轻，内质网趋于正常。结论：SSTF可下调皮质神经元Fas、FasL的表达，改善超微结构损伤，对脑I／R损伤有一定的保护作用。
Objective： To investigate the protective effects and mechanisms of scutellaria baicalensis stem-leave total flavonoid （SSTF） pretreatment on focal cerebral ischemia reperfusion injury in rat hippocampus. Methods： 30 SD rats were randomly divided into three groups as following： a sham operation group, an ischemia reperfusion group, and SSTF group. The rats in the SSTF group were perfused with SSTF, and the rats in the other two groups were given PBS solution instead of SSTF equally in a week before the operation. The model of focal cerebral ischemia reperfusion was established by the suture embolic method after treated with SSTF for 1 week. After 2 h of occlusion and 24 h of reperfusion, the morphological changes of the cortex neurons were observed. The expressions of Fas, FasL and ultrastructure in the neurons of the cerebral cortex were observed by immunohistochemical staining and transmision electron microscopy. Results： SSTF alleviated the pathological changes in the cerebral cortex. Compared with I/R group, the positive cells count of Fas and FasL were decreased significantly in the SSTF group; the shape of cells was relatively regular, mitochondria swelling decreased, endoplasmic reticulum and nuclear membrane tended to be normal and integrated. Conclusion： SSTF ameliorate cortex injury induced by focal cerebral ischemia reperfusion in rats, which may be related to down-regulation of Fas and FasL protein expression therefore improving the ultra-structure of the neurons.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2014年第1期47-50,共4页
Chinese Journal of Anatomy
基金
河北省科技厅,河北省教育厅资助课题（07276101D-46,2005227）
关键词
局灶性脑缺血再灌注
黄芩茎叶总黄酮
Fas
FASL
超微结构
focal cerebral ischemia reperfusion
scutellaria baicalensis stem-leaf total flavonoid
Fas
FasL
ultrastructure
