摘要
为研究血管内皮生长因子(vascular endothelial growth factor,VEGF)对绵羊卵母细胞体外成熟的影响。本试验仅在成熟培养阶段的不同培养液(血清培养体系fetal bovine serum,FBS和牛血清白蛋白培养体系albumin from bovine serum,BSA)中添加不同浓度VEGF(5和10 ng/mL),通过成熟率、卵裂率、桑葚胚率、囊胚率反映其影响效果。结果表明:FBS体系中,添加VEGF两试验组卵母细胞成熟率提高到84.61%和82.87%(P<0.01);BSA体系中,2试验组成熟率分别提高到82.40%和79.21%(P<0.01);无论是FBS体系还是BSA体系,添加VEGF的各个试验组成熟卵经受精后的胚胎发育都有不同程度的提高,且具有差异显著性。结论:VEGF在不同培养体系中,都可能是绵羊卵母细胞体外成熟的重要调节因子之一,且5 ng/mL VEGF为最适添加量;在FBS体系中,VEGF可能与存在于血清中的多种未知生长因子协同作用,更加有效的提高绵羊卵母细胞体外成熟率和胚胎发育潜力。
The effect of vascular endothelial growth factor （VEGF） on the ovine oocytes maturation was investigated in vitro.Experiments were conducted with the 5 and 10 ng/mL VEGF supplemented to the differmt maturation medium （the maturation medium with albumin from bovine serum,BSA and the maturation medium with fetal bovine serum,FBS）,resectivety.The effect of VEGF would be proved by maturation rate,cleavage rate and the development rates of morulae and blastocyst.The results showed that the maturation rate raised to 84.61％ and 82.87 ％ significantly （P＜0.01） in the two treatment group with FBS maturation medium,and increased to 82.40 ％ and79.21％ （P ＜0.01） with BSA maturation medium.Moreover the development rates of cleavage,mondae and blatocyst were all significantly improved in the two treatment group with VEGF.These results indicated that VEGF may be one of the important regular factors whether in system of maturation medium with FBS or BSA for ovine oocyte maturation in vitro,and 5 ng/mL additive amount of mecmbinant human VEGF165 was suitable.And in FBS maturation medium with VEGF,the rate of maturation and fertilization and consequently the capability of embryos development were markedly enhanced because of the synergistic effect between VEGF and other unknown growth factors in FBS.
出处
《西南农业学报》
CSCD
北大核心
2014年第1期402-407,共6页
Southwest China Journal of Agricultural Sciences
基金
国家自然科学基金项目(31060309)
中国博士后科学基金项目(2012M511579)
西北民族大学2012实验室开放基金项目
关键词
血管内皮生长因子
卵母细胞体外成熟
绵羊
Vascular endothelial growth factor （VEGF）
Oocytes maturation in vitro
Ovine
