摘要
目的探讨氯乙醇对鼠肝线粒体Ｃａ－Ｍｇ－ＡＴＰａｅ的体外毒性。方法采用游离线粒体和游离肝细胞系统，测定Ｃａ－Ｍｇ－ＡＴＰａｓｅ的活力及游离肝细胞大分子放射性。结果氯乙醇对小鼠肝线粒体钙泵的损害存在明显的剂量－反应关系及时间－效应关系；其抑制性质是属于不可逆抑制；１４Ｃ－氯乙醇与游离肝细胞大分子共价结合存在明显的时间依赖性。结论氯乙醇对鼠肝线粒体钙泵的不可逆抑制作用可能与共价结合有关。
Objective To investigate the toxic mechanism of 2-chloroethanol (CE) on mouse liver. Methods In vitro model was applied to comparative study on the variation of Ca-Mg-ATPase of mitochondria and on the possible covalent binding of CEmacromolecules. Results It manifested that CE did impair the calcium pump of mitochondria in the observable manner of both doseresponse relationship and time-effect relationship, and the damage seemed to be irreversible by the enzyme kinetics analysis. In the mean time covalent binding of 14C-labeled CE to macromolecules of cultured hepatocytes was also found in a time-dependent manner. Conclusion The damage of CE on the calcium pump of mitochondria might be irreversible because of the formation of covalent binding of CE-target macromolecules.
出处
《中国职业医学》
CAS
北大核心
2001年第1期22-23,共2页
China Occupational Medicine
