摘要
研究比较了来自正常和白化处理的皇家嘎拉 (RoyalGala)苹果 (MalusdomesticBorkh)新梢节段外植体不定芽的离体再生。结果表明 ,白化处理的新梢顶部第一节段外植体所产生的不定芽数比第二、第三、第四节段分别高 2、8、73倍 ;白化新梢的外植体不定芽再生数目比同一部位正常新梢高 7倍。培养基添加较低浓度的TDZ有利于不定芽再生 ,对白化外植体来说 ,1 μmol/L比 1 0 μmol/L高 2 .5倍 ,非白化外植体则高 7倍 ,平均每个外植体的不定芽再生数高达 68.8个。白化第一节段外植体的不定芽再生能力比常用的叶片外植体高 4倍 。
Studies on internodal explants compared from etiolated and non-etiolated shoots of ‘Royal Gala’apple (Malus domestic Borkh) showed that the first (youngest) internodal explant from etiolated shoots respectively produced 2,8 and 73 times of the number of adventitious buds compared to the second, third, or fourth internodal explant. Moreover, this explant produced 7 times compared to the similar explants from non-etiolated shoots. TDZ concentration influenced regeneration of internodal explants, with 1μM TDZ promoting about 2 and 7 times of the number of adventitious buds per explant from etiolated and non-etiolated stems respectively, compared to 10μM TDZ,and 68.8 adventitious buds were got from every explant in average. The regeneration ability of this explant was 4 times that of the other stem explants, so it could have application in apple gene transfering and genome breeding.
出处
《落叶果树》
2001年第1期4-7,共4页
Deciduous Fruits
基金
山东省科委农业"三O"工程课题内容之一
