摘要
目的 观察我国首例活体部分小肠移植受体围手术期趋化因子 IL- 8的变化及其与移植物缺血再灌注损伤和急性排斥反应的相关性 .方法 双抗夹心 EL ISA法测定受体术前及术后 1～ 140 d血清 IL- 8动态水平 .结果 受体血清 IL- 8水平在术后 1d显著升高 (2 .44 μg· L- 1 vs0 .2 6 μg· L- 1 ) ,且于次日迅速下降 (1.70 μg· L- 1 ) ,术后 4d达术前水平 .血清 IL- 8的第 2个高峰出现于术后 6 7d发生移植物急性排斥反应时(1.47μg· L- 1 ) ,但高度较第 1次低 ,恢复较第 1次慢 ,在术后 96 d达术前水平 .结论 在人活体部分小肠移植中 ,血清IL- 8水平与移植物缺血再灌注损伤和急性排斥反应密切相关 ,可作为重要的小肠移植物急性排斥反应的信号之一 .
AIM To investigate the perioperative serum levels of IL-8 in the first case of living-related small bowel transplantation in China and to study the interrelationship of the changes in serum IL-8 level with ischemic and reperfusive injury and with the acute small bowel allograft rejection. METHODS Dynamic serum levels of IL-8 of recipient were analyzed by a solid-phase double ligand ELISA method once preoperation and several times during 1~140 d postoperation. RESULTS Recipient's IL-8 serum level increased on 1 postoperatior day (POD) (2.44 μg·L -1 vs 0.26 μg·L -1), decreased rapidly the next day (1.70 μg·L -1), and returned to the level of preoperation at 4 POD. Its second peak level occurred on 67 POD with acute small bowel allograft rejection (1.47 μg·L -1). Then it decreased slowly and returned to the level of preoperation on 96 POD. CONCLUSION In the human living-related small bowel transplantation, there is a close correlation of serum IL-8 levels with ischemic and reperfusive injury and with the acute small bowel allograft rejection. It could be regarded as one of the most important signs of acute small bowel allograft rejection.
出处
《第四军医大学学报》
北大核心
2001年第2期131-134,共4页
Journal of the Fourth Military Medical University
关键词
活体供者
小肠移植
白细胞介素8
移植物排斥
living donnors
intestine, small/transplantation
interleukin-8
graft rejection
