摘要
目的 建立能同时测定尿液中香草扁桃酸（VMA）、5-羟吲哚乙酸（5-HIAA）、3,4-二羟苯乙酸（DOPAC）和高香草酸（HVA）4种生物胺代谢物的高效液相色谱法.方法 尿样经0.45 μm过滤头过滤后直接进样,采用C18色谱柱分离,荧光检测器检测,波长：EX 280 nm,EM 315 nm,以甲醇-0.1 mol/L磷酸盐缓冲液（体积比20：80）为流动相,流速为1.0 ml/min,进样体积为20 μl.结果 该方法测定VMA、5-HIAA、DOPAC、HVA 4种物质线性范围分别为0～2.5、0～2.0、0～2.0、0～2.5 μg/ml；相关系数分别为0.999 9、0.999 8、0.999 7和0.999 9；最低检出浓度分别为0.006、0.008、0.001 2和0.008 2 μg/ml;平均精密度分别为4.2％、3.7％、4.9％和3.6％；回收率分别为91％～102％、93％～101％、94％～101％、89％～102％.结论 该方法简便、高效、准确,适用于尿液VMA、5-HIAA、DOPAC、HVA含量的同时测定.
Objective To establish a method for simultaneously determiniug vanilmandelic acid （VMA）,5-hydroxyindoleacetic （5-HIAA）,3,4-dihydroxyphenylacetic acid （DOPAC）,and homovanillic acid （HVA） in urine by high-performance liquid chromatography （HPLC）.Methods After being filtered with a 0.45 μm membrane syringe filter,the urinary samples were injected directly into the HPLC system using a C18 chromatographic column and a fluorescence detector.The excitation and emission wavelengths were chose as 280 nm and 315 nm,respectively,and the urinary samples were carried with a mobile phase of methanol-0.1 mol/L phosphate buffered solution （V/V=20：80） at a flow rate of 1.0 ml/min and an injection volume of 20 μ1.Results Using the method reported here,the correlation coefficients of VMA,5-HIAA,DOPAC,and HVA were 0.9999,0.9998,0.9997,0.9999,respectively,over linear ranges of 0-2.5,0-2.0,0-2.0,and 0-2.5 μg/ml,the minimum detectable concentrations were 0.006,0.008,0.012,and 0.0082 μg/ml,the average precisions were 4.2％,3.7％,4.9％,and 3.6％,and the recovery rates were 91％～102％,93％～101％,94％～101％,and 89％～ 102％.Conclusion This determination method is simple,efficient,accurate,and sensitive for the simultaneous detection of VMA,5-HIAA,DOPAC,and HVA in urine.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2014年第2期140-142,共3页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
河南省医学科技攻关计划重大项目（201001009）
河南省卫生科技创新型人才工程科技领军人才基金（3022）
关键词
香草扁桃酸
5-羟吲哚乙酸
3
4-二羟苯乙酸
高香草酸
色谱法
高效液相
Vanilmandelic acid
5-Hydroxyindoleacetic
3,4-Dihydroxyphenylacetic acid
Homovanillic acid
High-performance liquid chromatography
