摘要
目的 探讨巨噬细胞衍生趋化因子（MDC）基因多态性与支气管哮喘发病的关系.方法 采用放大受阻突变系统,分别检测341例哮喘患者及392例健康对照者MDC基因启动区-469（A/G）、-721（C/T）、-942（C/T）三个基因多态性（SNPs）,采用ELISA法测定血清MDC及总IgE浓度.根据哮喘患者发病年龄特点将哮喘患者分成13岁以下组和13岁以上组.结果 校正年龄和性别后,13岁以上哮喘患者组MDC-469（A/G）SNP各基因型分布和对照组间差异有统计学意义[P＝0.046,odds radio：1.28（1.0-1.63）].在哮喘患者组及对照组均发现MDC-942（C/T）SNP各基因型间血清MDC水平差异有统计学意义（哮喘组：P＝0.035;对照组：P＝0.010）,在哮喘患者组MDC-942（C/T）SNP各基因型间血清总IgE水平差异有统计学意义（P＝0.016）.结论 MDC-469（A/G）SNP可能是支气管哮喘发病风险因子,MDC-942（C/T）SNP影响哮喘患者血清总IgE水平,MDC SNPs可能是支气管哮喘发病相关的遗传子之一.
Objective To investigate the clinical significance of the single nucleotide polymorphisms （SNPs） in the macro- phage-derived chemokine （MDC） gene promoter in asthma. Methods Genotyping of three SNPs, -469 （A/G）, -721 （C/T） , and - 942（ C/T）, in the MDC gene promoter was performed using the amplification refractory mutation system （ARMS） in 341 patients with asthma and 392 control subjects. Levels of MDC and total IgE in serum were measured by specific enzyme-linked immunosorbent assay kits. The study subjects were divided into two subgroups by age of onset： more than 13 years old group and less than 13 years old group. Results There was significant difference in the genotype distribution of MDC-469 （A/G） SNP between controls and more than 13 years old patients [ P =0. 046, odds radio： 1.28 （ 1.0 - 1.63） ] adjusted by sex and age. Serum levels of MDC were significantly different among three genotypes of CC, CT, and TT of MDC-942（C/T） SNP in patients with asthma （ P =0. 035） and in controls （ P = 0. 010）. Serum total IgE levels, similarly, were significantly different among three genotypes of MDC-942（C/T） SNP in asthmas （ P -0. 016）. Conclusions MDC-469 （A/G） SNP in MDC gene promoter was a risk factor in asthma, MDC-942 （C/T） SNP influ- enced IgE levels of serum in asthmas. MDC gene may be one of correlative genes in asthmas.
出处
《中国医师杂志》
CAS
2014年第1期37-41,共5页
Journal of Chinese Physician
