摘要
为了检验水稻基因组分子标记在谷子基因组中的可转移性及多态性,选取59份不同的谷子材料,利用基于水稻基因组数据设计的38个扩增共有序列遗传标记(ACGM)进行分析,并采用DARwin5.0.156软件对供试材料进行了分子聚类分析。结果表明:33对引物可以在至少5份以上谷子材料中获得扩增产物,占引物总数的87%。其中,在供试材料之间有多态性的引物共7对,占引物总数的18.4%。供试材料的分子聚类结果表明,采用水稻ACGM标记进行的聚类并没有按谷子的表型数据或生态类型聚在一起,其原因尚需进一步研究。这些结果表明基于水稻基因组序列开发的ACGM标记在谷子中具有较高的可转移性,但与其他作物相比,这些ACGM标记在谷子中的多态性偏低。
The purpose of the present research was to study the transferability and the polymorphism of rice（Oryza sativa） genome data in foxtail millet（Setaria italica）. 38 ACGM markers exploited from rice were investigated in 59 foxtail millet accession using PCR methods. The transferability and the polymorphism of rice（Oryza sativa） genome data in foxtail millet were analyzed and the polymorphism data of these markers among the accession were used for clustering through biostatistics DARwin 5.0.156. The results showed that a total of 33 pairs of primers behaved specific PCR products in at least five accessions, about occupying 87% of total primers. Among those, 7 pairs of primers occupying 18.4% of the total markers were of polymorphism among the accessions. The molecular clustering results of the tested materials showed that it was not fully consistent with that depending on the phenotypic or ecological data and the reason need further study. The results showed that the makers from rice had a high transferability in foxtail millet and the polymorphism of these markers was lower in foxtail millet compared with other crops.
出处
《中国农学通报》
CSCD
2014年第3期219-225,共7页
Chinese Agricultural Science Bulletin
基金
山西农业大学作物学博士后科研流动站"谷子高度雄性不育系高146A雄性不育基因和抽穗期QTL的分子标记定位"(92948)
山西省自然基金"谷子SSR(EST-SSR)遗传连锁图谱的构建"(2012011032-1)
国家谷子糜子产业技术体系资助(CARS-07-12.5-A10)
关键词
谷子
水稻
ACGM标记
标记可转移性
多态性
foxtail millet
rice
ACGM marker
transferability of markers
polymorphism
