摘要
稀有海洋放线菌Salinispora arenicola CNH643DSM 44819的sare4854基因编码1个典型的SARP,但其确切功能没有得到认证.为了研究sare4854基因的功能,我们在链霉菌Streptomyces sp.AM-7161中异源表达了sare4854基因.实验结果表明,对比野生菌,转化子中抗生素的产量被明显抑制.生物信息学分析表明,sare4854除了与编码SARPs蛋白家族中的其他1样在N-端编码1个SARP样结构域外,在其C-端还编码有1个核苷三磷酸水解酶(nucleoside triphosphate hydrolases,NTPase)结构域.这可能是sare4854基因对Streptomyces sp.AM-7161抗生素产量负调控的主要作用机制.
Streptomyces antibiotic regulatory proteins （SARP） normally play positive regulatory roles during streptomyces antibiotics biosynthesis.sare4854,a gene in Salin ispora arenicola CNH643 DSM 44819 was proposed to encode a SARP.Here,sare4854 was expressed in a heterolohous system for functional characterization.However,the antibiotic production in resultant strain was repressed significantly.Bioinfomatic analysis showed that sare4854 has an additional nucleoside triphosphate hydrolases （NTPase） domain at the C-terminus,besides a SARP-like domain （DBD：DNA binding domain and BTA：bacterial transcriptional activation domain） at the N-terminus conserved in all SARPs.The possible mechanism of sare4854 in antibiotic synthesis in this abnormal phenomenon was discussed here.
出处
《华中师范大学学报（自然科学版）》
CAS
北大核心
2014年第1期75-79,85,共6页
Journal of Central China Normal University：Natural Sciences
基金
国家自然科学基金项目(20772040.)
中央高校自主项目(CCNU11A02013)
湖北省遗传调控与整合重点实验室项目(GRIB201204)
