摘要
目的:提取构橘叶挥发性成分,进行抗菌抗肿瘤活性测试,为进一步研究开发构橘提供实验依据。方法:利用水蒸气蒸馏法制备构橘叶挥发油,计算收率,并结合GC-MC分析化学成分;用纸片琼脂扩散法和MTT比色法分别研究构橘叶挥发油抗菌和抗肿瘤活性。结果:水蒸气蒸馏法提取构橘叶挥发油收率为0.78%,得到65个化合物,鉴定出51个,占总成分的91.25%;构橘叶挥发油对枯草杆菌具有较好的抑菌活性,对白血病细胞(K-562)、肝癌细胞(BEL-7402)具有一定的抑制活性,IC50分别为31.53和36.84 g·mL-1。结论:构橘叶挥发油的主要成分为芳樟醇氧化物(11.93%)、蓝桉醇(10.18%)、喇叭茶萜醇(8.92%)等。抗菌实验结果表明构橘叶挥发油对枯草杆菌具有较好的抗菌活性。抗肿瘤活性实验结果显示构橘叶挥发油对白血病细胞K-562和肝癌细胞BEL-7402细胞具有抑制增殖的活性。
Objective：To study the chemical constituents and bioactivity of essential oil from Poncirus trifoliata.Method：The essential oil was extracted by hydrodistillation and separated by capillary GC.The chemical constituents were determined by normalization and were identified by MS.Result：65 chromatographic peaks were detected and 51 compounds were identified,which were 91.25％ of the total essential oil.The cytotoxicity of the essential oil against human cancer cell lines BEL-7402,K-562,SGC-7901 and SPCA-1 was investigated in vitro.Conclusion：The major components of the essential oil were linalool oxide （11.93％）,（-） globulol （10.18％） and Ledol （8.92％）.The oil showed moderate activity against K-562 and BEL-7402 human tumor cell lines with IC50 values less than 40 μg/ml.The oil was also found to possess antimicrobial activity against Bacillus subtilis.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第5期97-101,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
新世纪优秀人才项目(NCET-08-0656)
海南省中药现代化基金项目(2011ZY010)
关键词
枸橘
挥发油
化学成分
气相色谱-质谱
Poncirus trifoliata
essential oil
chemical constituent
GC-MS
