摘要
目的：建立同时测定唇形科植物独一味、糙苏、螃蟹甲和萝卜秦艽中3个苯乙醇苷成分（acteoside、alyssonoside、isoacteoside）含量的方法。方法：采用高效液相色谱法。色谱柱为SymmetryC18（150mm×4．6mm，5μm），流动相为乙腈-水（用醋酸调pH为4．5，梯度洗脱），流速为1．0ml／min，柱温为20℃，检测波长为330nm。结果：acteoside、alyssonoside、isoacteoside的质量浓度分别在20-620、5～125、5～125μg／ml范围内与各自峰面积积分值呈良好线性关系（，分别为0．9994、0．9998、0．9997）；精密度、稳定性、重复性试验的RsD〈5％；平均加样回收率分别为101．15％、96．98％、102．01％，RSD分别为3．41％、3．64％、1．80％（n均为6）。结论：该方法简便、准确、重复性好，可用于测定独一味、糙苏、螃蟹甲和萝卜秦艽中3个苯乙醇苷的含量。
OBJECTIVE： To establish the method for the content determination of 3 kinds of phenylethanoid glycosides （acteoside,alyssonoside, isoacteoside） in Lamiophlomis rotate, Phlomis umbrosa, Phlomis younghusbandii and Phlomis medicinaliss. METH- ODS： HPLC method was adopted. The determination was performed on Symmetry C18 （150 mm× 4.6 mm, 5 μm） column with mobile phase consisted of acetonitrile-water （pH adjusted to 4.5 using acetic acid, gradient elution） at the flow rate of 1.0 ml/min. The column temperature was 20 ℃ and the detection wavelength was set 330 nm. RESULTS： The linear ranges of acteoside, alyssonoside, isoacteo- side were 20-620 μg/ml（r=0.999 4）, 5-125μg/ml（r=0.999 8） and 5-125 μg/ml（r=0.999 7）, respectively. RSDs of precision, stabili- ty and reproducibility tests were all lower than 5%. The average recoveries of them were 101.15% （RSD=3.41%, n=6）, 96.98% （RSD=3.64% ,n=6） and 102.01% （RSD= 1.80% ,n=6）. CONCLUSIONS： The method is simple, accurate, reproducible and suit- able for the content determination of 3 kinds of phenylethanoid glycosides in L. rotate, P. umbrosa, P. younghusbandii and P. medicinaliss
出处
《中国药房》
CAS
CSCD
2014年第11期1027-1029,共3页
China Pharmacy
基金
甘肃省科技重大专项计划项目(No.1102FKDA012)
关键词
独一味
糙苏
螃蟹甲
萝卜秦艽
苯乙醇苷
反相高效液相色谱法
含量测定
Lamiophlomis rotata
Phlomis umbrosa
Phlomis younghusbandii
Phlomis medicinalis
Phenylethanoid glycosides
RP-HPLC
Content determination
