摘要
【目的】研究铜(Cu2+)胁迫条件下亚洲玉米螟(Ostrinia furnacalis Guenée)亲代及其子代的生长发育与生殖,为亚洲玉米螟的种群动态预测与防治提供依据。【方法】以亚洲玉米螟幼虫为材料,在标准人工饲料中添加不同浓度(25、50、100、200和500 mg·kg-1)的重金属Cu2+,在实验室条件下连续饲养亚洲玉米螟幼虫3代,系统观察重金属Cu2+胁迫对亚洲玉米螟亲代及其子代生长发育与生殖的影响。【结果】亚洲玉米螟的幼虫存活率、化蛹率和成虫羽化率与Cu2+胁迫浓度呈显著负相关,引起亲代、子一代和子二代幼虫存活率和成虫羽化率显著下降的最低Cu2+胁迫浓度分别为100、50、50 mg·kg-1,引起各代化蛹率显著下降的最低Cu2+胁迫浓度分别为200、50、50 mg·kg-1,其中Cu2+浓度200 mg·kg-1及其以上浓度处理的子一代和子二代幼虫死亡率高达20%以上,Cu2+浓度500 mg·kg-1处理的各代幼虫化蛹率均低于80%,Cu2+浓度200 mg·kg-1处理的子二代成虫羽化率低于50%。亚洲玉米螟的幼虫体重和蛹重与Cu2+胁迫浓度呈极显著负相关,引起各代幼虫体重显著下降的最低Cu2+胁迫浓度均为50 mg·kg-1,引起亲代、子一代和子二代蛹重显著下降的最低Cu2+胁迫浓度分别为500、50、50 mg·kg-1,其中Cu2+浓度50 mg·kg-1及其以上浓度处理的各代3龄、5龄幼虫的体重显著低于空白对照;Cu2+浓度500 mg·kg-1处理的亲代蛹重显著低于对照,其他Cu2+浓度处理的蛹重与对照无显著差异;Cu2+浓度50 mg·kg-1及其以上浓度处理的子一代和子二代蛹重显著低于对照,Cu2+浓度25 mg·kg-1处理的蛹重与对照无显著差异。亚洲玉米螟各代幼虫历期和蛹历期与Cu2+胁迫浓度呈显著正相关,其中Cu2+浓度50 mg·kg-1及其以上浓度处理的各代幼虫历期显著高于对照,Cu2+浓度200 mg·kg-1及其以上浓度处理的亲代蛹历期、Cu2+浓度100 mg·kg-1及其以上浓度处理的子一代蛹历期和Cu2+浓�
【Objective】The objective of this study is to assess the effects of copper (Cu2+) stress on the growth and reproduction of Ostrinia furnacalis Guenée in the parental generation and its filial generation, and to provide a basis for the forecast of population dynamics of O. furnacalis and its control. 【Method】O. furnacalis larvae in three generations were continuously fed with standard artificial diet containing different concentrations of Cu2+ (25, 50, 100, 200 and 500 mg·kg-1) in the laboratory and the effects of copper (Cu2+) stress on the growth and reproduction of O. furnacalis were investigated.【Result】The larval survival rate, pupation rate and eclosion rate of O. furnacalis were significantly negatively correlated to the concentration of Cu2+ stress. The minimum concentrations of Cu2+ at 100, 50 and 50 mg·kg-1 were significantly decreased the survival rate and eclosion rate in the parental generation, the first and second filial generation, respectively, and 200, 50 and 50 mg·kg-1 were significantly decreased the pupation rate in each generation, respectively, which the larval mortality rates of the treatments at the concentrations of Cu2+ 200 mg·kg-1 and higher than 200 mg·kg-1 in the first and second filial generation were higher than 20%, the pupation rates of the treatment at the concentration of Cu2+ 500 mg·kg-1 in each generation were lower than 80%, the eclosion rate of the treatment was lower than 50% at the concentration of Cu2+ 200 mg·kg-1 in the second filial generation. The larval and pupal body mass of O. furnacalis were significantly negatively correlated to the concentration of Cu2+ stress. The minimum concentration of Cu2+ at 50 mg·kg-1 was significantly decreased the larval body mass in each generation, and 500, 50 and 50 mg·kg-1 were significantly decreased the pupal body mass in the parental generation, the first and second filial generation, respectively, which the body mass of the third and fifth instar larvae of the treatments at con
出处
《中国农业科学》
CAS
CSCD
北大核心
2014年第3期473-481,共9页
Scientia Agricultura Sinica
基金
江西省自然科学基金(20114BAB204010)
关键词
重金属
铜
亚洲玉米螟
生长发育
生殖
heavy metal
copper
Ostriniafurnacalis Guenee
growth
reproduction