摘要
目的探讨鞣花酸对乳腺癌细胞MDA-MB-231的增殖侵袭转移的作用。方法采用0(对照)、6、12μg/ml鞣花酸培养液分别处理乳腺癌细胞MDA-MB-231,分别于培养后24、48、72 h计数MDA-MB-231细胞数。细胞趋化实验观察鞣花酸对MDA-MB-231细胞趋化运动的影响,Western Blot观察鞣花酸对乳腺癌细胞MDA-MB-231中SDF-1α信号通路激活的抑制作用。数据分析采用重复测量的方差分析,两两比较采用SNK-q分析方法。结果与对照组比较,6、12μg/ml鞣花酸处理组在24、48、72 h的细胞计数显著降低。重复测量的方差分析结果提示分组比较(F=4875.56,P=0.00)及三个时间点间比较(F=670.73,P=0.00)差异有统计学意义,而分组与时间有交互作用(F=122.92,P=0.00),表明鞣花酸对乳腺癌MDA-MB-231细胞增殖有显著抑制作用。乳腺癌细胞趋化运动实验提示各组乳腺癌细胞的趋化数分别为(14.00±1.00)×105/ml、(7.70±0.58)×105/ml、(3.00±1.00)×105/ml,差异有统计学意义(F=117.57,P=0.00)。Western Blot结果显示鞣花酸明显抑制CXCR4表达及SDF1α/CXCR4对乳腺癌细胞AKT信号通路的激活。结论鞣花酸可抑制乳腺癌MDA-MB-231细胞增殖,SDF1α/CXCR4介导的细胞趋化运动及其SDF1α/CXCR4信号通路激活,在预防乳腺癌复发及转移中可能有潜在价值。
Objective To investigate the effect of ellagic acid on proliferation and invasion and metastasis of breast cancer cells MDA-MB-231. Methods The MDA-MB-231 cells were cultured and treated with 0（ control group） , 6,12 μg/ml ellagic acid respectively, then the cells were counted at 24, 48, 72 h after culture. Chemotaxis experiment and Western Blot were conducted to observe the effects of eilagic acid on chemotaxic movement of MDA-MB-231 cells and SDF-lα signaling pathway activation. The data were analyzed using repeated measurement analysis of variance, and pairwise comparison was performed using the SNK-q analysis. Results Compared with the control group, cell counts in 6, 12 μg/ml ellagic acid treatment groups were significantly reduced at 24, 48, 72 h. Repeated measurement analysis of variance indicated that the comparison between groups （ F = 4875.56, P = 0. 00） and among the three time points （ F= 670. 727, P = 0. 00） showed a statistically significant difference and the interaction existed between group and time（ F= 122. 92, P= 0. 00）. It showed that ellagic acid can significantly inhibit the proliferation of breast cancer cells MDA-MB- 231. Chemotaxis experiment of breast cancer cells showed that chemotaxic cells were （ 14. 00± 1.00 ） × 10^5/ml, （7.70±0. 58 ） × 10^5/ml, （3.00± 1.00） × 10^5/ml in each group respectively, and the difference was statistically significant （F= 117.57, P= 0. 00 ）. Western Blot result showed that ellagic acid significantly inhibited CXCR4 expression and activation of AKT signaling pathway by SDF1α/CXCR4 in breast cancer cells. Conclusion E1iagic acid can inhibit the proliferation and SDF1α/CXCR4 mediated chemotaxic movement of breast cancer cells MDA-MB-231 and regress the activation of SDF1 α/CXCR4 signaling pathway, which may have potential value in the prevention of breast cancer recurrence and metastasis.
出处
《中华乳腺病杂志（电子版）》
CAS
2013年第6期20-23,共4页
Chinese Journal of Breast Disease(Electronic Edition)
基金
十堰市太和医院院级项目(2012TTXM01)
