摘要
目的:建立高效液相色谱法测定己二烯雌酚片的有关物质。方法:采用Hypersil C18色谱柱(250 mm×4.6 mm,5μm),甲醇-乙腈(76∶24)为流动相,流速:0.8 ml·min-1,检测波长:229 nm,进样量:10μl。结果:在选定的色谱条件下,己二烯雌酚与有关物质分离完全,最低检测限为2 ng。结论:该方法简便、准确、专属性好、灵敏度高,可用于己二烯雌酚片的有关物质测定。
Objective： To determine the related substances in dienestrol tablets by HPLC. Methods： The separation was performed on a C18 column （250 mm×4.6mm,5μm）. The mobile phase consisted of methanol-acetonitrile （76：24）, the flow rate was set at 0.8 ml·min-1 with the detection wayelength at 229 nm, and the sample size was 10 μl. Results： Under the established chromatographic conditions, the related substances and dienestrol were separated completely. The limit of detection was 2 ng. Conclusion： The method is simple, accurate, specific and sensitive in the determination of related substances in dienestrot tablets.
出处
《中国药师》
CAS
2014年第2期341-343,共3页
China Pharmacist
