摘要
目的:探讨补充天然虾青素和游泳训练对大鼠骨骼肌抗氧化能力的影响。方法:32只7周龄健康雄性SD大鼠适应性喂养3天后按体重随机分为4组。即对照组(C)、运动组(T)、服药组(M)和运动服药组(TM)。T组和TM组进行6周的递增负荷游泳运动,M组和TM组每天灌服天然虾青素,剂量为400mg/Kg BW。6周后,分别测定各组大鼠血液中肌酸激酶和乳酸脱氢酶含量,测定骨骼肌细胞中超氧化物歧化酶、丙二醛、谷胱甘肽过氧化物酶和过氧化氢酶含量。结果:长期递增负荷训练使大鼠血清LDH(P<0.01)和CK(P<0.01)水平显著上升,骨骼肌SOD活性、SOD/MDA比值、GSH-PX、T-AOC和CAT显著降低。补充天然虾青素显著降低了长期递增负荷训练大鼠血清的LDH、CK浓度和骨骼肌MDA的含量,同时显著提高骨骼肌SOD活性、SOD/MDA比值,GSH-PX、T-AOC和CAT活性。结论:①长期递增负荷训练使骨骼肌细胞膜结构和功能的完整性遭到了破坏,主要表现为血清LDH和CK浓度的增加。②补充天然虾青素显著降低了长期递增负荷训练大鼠血清的LDH、CK浓度和骨骼肌MDA的含量。骨骼肌SOD活性、SOD/MDA比值,GSH-PX、T-AOC和CAT显著增高,这可能是天然虾青素保护运动机体骨骼肌的细胞膜功能和结构完整性的机制。
Purpose： The purpose of the research is to discuss the effect of gavaging astaxanthin combined with quantitative load swimming training on antioxidant capacity in rats＇skeletal muscle. Methods;32 healthy male SD rats of 7 weeks old are selected and fed adaptively for three days and divided into 4 groups randomly ： control group （ C）, exercise group（T）, medication group（M） and exercise medicine -taking group （TM）. The exercise group and exercise medicinetaking group undergo progressive load swimming exercise for 6 weeks, The treated group and the control group were gavaged natural astaxanthin daily, a dose of 400mg/kg. Detection of rat blood Creatine kinase , lactate de- hydrogenase content, skeletal muscle tissue superoxide dismutase, malondialdehyde, glutathione peroxidase, catalase content is conducted after 6 weeks swimming exercise. Results ： long - term increasing load training significantly increa- ses rats＇serum LDH （P 〈 0.01） and CK （P 〈 0.01 ） levels, while skeletal muscle SOD activity, SOD/MDA ratio, GSH -PX , T- AOC and CAT decrease significantly. Gavaging natural astaxanthin reduces serum LDH, CK concentration and skeletal muscle MDA content induced by long -term increasing load training, but at the same time signifi- cantly increases the skeletal muscle SOD activity, SOD/MDA ratio, GSH - PX, T - AOC and CAT. Conclusions ： （ 1 ） longterm increasing load training damages the structure, function and integrity of the skeletal muscle cell membrane, which is mainly reflected in the increase of concentration of serum LDH and CK. （2） Gavaging natural astaxanthin reduces serum LDH , CK concentration and skeletal muscle MDA content induced by long -term increasing load training. The SOD activity in skeletal muscles, SOD/MDA ratio, GSH- PX, T- AOC and CAT increase significantly, which might be the mechanism of natural astaxanthin to protect the function and structural integrity of the cell membrane in movement skeletal muscles
出处
《成都体育学院学报》
CSSCI
北大核心
2014年第2期75-79,共5页
Journal of Chengdu Sport University
基金
江苏省教育厅自然科学基金项目(IK040192)
关键词
天然虾青素
骨骼肌
自由基代谢
抗氧化能力
natural astaxanthin
skeletal muscle
free radical metabolism
antioxidant
