摘要
优化阴香花中原花青素的提取工艺。以经冷冻干燥、粉碎与80目过筛的阴香花粉末为供试物料,丙酮-乙醇混合提取,提取液以铁盐催化显色,测定反应体系的吸光度。在提取溶剂体积分数、pH值、料液比、温度及时间单因素试验基础上进行正交试验优化提取条件。结果表明:以体积分数70%的混合液(70%丙酮-70%乙醇(3∶2))为提取溶剂、pH 2、料液比1∶7.5(g/mL)、提取温度50℃、提取时间2 h提取阴香花原花青素的效果为佳,以此参数条件下提取3次原花青素的提取率为95.37%、得率为9.02%。丙酮是阴香花原花青素提取的有效溶剂,采用优化提取条件可有效的提取阴香花原花青素。
This study was undertaken to optimize the extraction of proanthocyanidins from flowers of Cinnamomum burmannii. The starting raw material, freeze-dried flowers of Cinnamomum burmannii ground and sieved through a 80- mesh sieve, was extracted using acetone-ethanol and the extract was submitted to color development catalyzed by iron salt before measuring the absorbance. Five extraction conditions including extractant concentration, pH, solid-to-solvent ratio, temperature and time were optimized by one-factor-at-a-time and orthogonal array design methods. The optimal extraction efficiency was obtained by three repeated cycles of extraction at 50 ℃ for 2 h using an extractant consisting of 70% acetone- ethanol in water, as prepared by mixing 70% acetone and 70% ethanol with a volume ratio of 3：2, with a solid-to-solvent ratio of 1：7.5 （g/mL）. Under these conditions, the proanthocyanidin recovery was 95.37%, and the proanthocyanidin content in dried flowers of Cinnamomum burmannii was 9.02%. Thus acetone is an effective solvent for extracting proanthocyanidins from flowers of Cinnamomum burmannii under optimized conditions.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2014年第2期115-119,共5页
Food Science
基金
广东省科技计划项目(2009B011300015)
关键词
阴香花
原花青素
提取工艺
flower of Cinnamomum burmannii
proanthocyanidins
extraction process
