摘要
目的了解河北省分离的9株B群脑膜炎奈瑟菌(Neisseria meningitides,Nm)的药物敏感性及分子流行病学特征。方法对分离菌株进行E-test法药物敏感性试验和多位点序列分型(Multilocus Sequence Typing,MLST)分析。结果药敏试验显示,9株B群Nm均对磺胺类抗生素耐药;6株分离自健康人的Nm中2株对喹诺酮类抗生素耐药;3株分离自流脑病人的Nm中的2株菌株对喹诺酮类抗生素耐药,且对青霉素类抗生素的敏感性降低。显示9株Nm菌株中的6株属于ST-4821克隆群,1株属于ST-175克隆群,2株无相应的克隆群。结论高致病性ST-4821已成为河北省B群Nm的优势克隆群,提示应加强流脑的病原学监测。
Objective To study the characteristics of drug susceptibility and molecular typing of 9 Neisseria meningiti- des serogroup B strains isolated in Hebei Province. Methods Isolates were subjected to a drug susceptibility test using the E-test method and multilocus sequence typing （MLST） was performed based on 7 house-keeping genes. Results Drug susceptibility testing indicated that the 9 N. meningitides serogroup B strains were resistant to sulfa antibiotics; 2 i- solates from healthy carriers were resistant to quinolone antibiotics and 2 isolates from patients were resistant to quinolone antibiotics and had reduced susceptibility to benzylpenicillin antibiotics. Six strains belonged to the ST-4821 clonal com- plex while 1 strain belonged to the ST-175 elonal complex. There was no corresponding clonal complex for the remaining 2 strains. Conclusion The ST 4821 clonal complex is highly pathogenic and has become the predominant clonal complex in Hebei Province. This indicates that monitoring of epidemic cerebrospinal meningitis should be enhanced.
出处
《中国病原生物学杂志》
CSCD
北大核心
2014年第1期65-67,共3页
Journal of Pathogen Biology
基金
河北省科技厅项目(No.11276137)
关键词
脑膜炎奈瑟菌
B群
药敏试验
多位点序列分型
Neisseria meningitides
serogroup B
drug susceptibility test
multilocus sequence typing
