摘要
目的：建立拉曼光谱法测定氯膦酸二钠注射液的含量的方法。方法：利用拉曼散射原理，在水溶液中，拉曼峰信号的强度与溶质分子的浓度成正比，选取样品溶液拉曼光谱中氯膦酸二钠的P=O基团振动（1079 cm^-1）处的强峰作为定量峰，建立拉曼光谱法测定氯膦酸二钠注射液含量的方法。结果：氯膦酸二钠在2-100 mg·mL^-1范围内与拉曼峰强度呈良好的线性关系，线性回归方程为Y=426.0X＋286.6，相关系数为0.9999（n=8），平均回收率为102.8%，RSD为1.6%（n=9）。结论：本方法操作简便、快速，可用于氯膦酸二钠注射液的含量测定。
Objective： To establish a Raman method for the determination of clodronate disodium injection. Methods： Based on the Raman scattering theory that the light intensity of the laser Raman peak is in proportional to the concentration of clodronate disodium samples in aqueous solution, a quantitative method for determination of clodronate disodium injection using Raman spectroscopy was established by selecting the Raman peak of clodronate disodium P=O bond （P=O[1079 cm^-1]） as the quantitative peak. Results： The calibration curve was linear over the range of 2-100 mg·mL^-1 （r= 0.9999,n=8）,and the average recovery was 102.8% with RSD of 1.6% （n=9）. Conclusion： The method is convenient and rapid,which can be used for the quality control of clodronate disodium injection.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2014年第2期364-367,共4页
Chinese Journal of Pharmaceutical Analysis
关键词
激光拉曼光谱
氯膦酸二钠
注射剂
含量测定
专属性
laser Raman spectra
clodronate disodium
injection
determination
specialization
