摘要
目的探讨普萘洛尔对增殖期血管瘤的治疗作用及其机制。方法将手术切除的婴幼儿增殖期血管瘤新鲜组织块移植于裸鼠皮下，建立血管瘤动物模型；在移植后的第45天，将移植瘤组织均成活的4（）只实验裸鼠随机分为2组：普萘洛尔治疗组和生理盐水对照组，每组20只，分别灌注普萘洛尔溶液和生理盐水治疗。每7d观察一次移植瘤的生长情况和测量肿瘤体积。在首次药物干预后的第7、14、21、28天，每组分别随机处死5只裸鼠，切取移植的瘤体组织各10个。病理组织切片观测肿瘤的病理组织学改变；免疫组化法检测移植瘤组织中血管内皮祖细胞标志物CDl33及细胞增殖核抗原Ki-67的表达情况；PCR检测瘤体组织中CDl33和CD31表达水平。结果药物干预后14d开始，普萘洛尔组瘤体体积小于生理盐水组（70．6±4．5比93．4±6．3）mm3（P〈0．05）。免疫组化检测第28天时普萘洛尔组瘤体的CDl33和Ki-67阳性细胞百分率明显低于第七天时（35．6±1．8比65．4±4．5；8．8±0．7比45．4±6．1）（P〈0．01）。PCR检测普萘洛尔组的CDl33水平逐渐降低，CD31水平逐渐升高（0．59±0．05，0．20±0．02；0．30±0．03，1．25±0．02）。而生理盐水组免疫组化CDl33、Ki-67阳性细胞百分率（65．4±5．1，45．4±6．4）及CDl33、CD31PCR检测值（0．59±0．05，0．30±0．03）均无明显改变（P〉0．05）。结论本实验显示普萘洛尔对血管瘤移植模型中的增殖期血管瘤有一定的治疗作用。其早期细胞机制与促进瘤体中内皮祖细胞向成熟内皮细胞转化有关。
Objective To explore the therapeutic effects and cellular mechanism of propranolol in the treatment of proliferating hemangioma. Methods Hemangioma model in nude mice was estab- lished through fresh hemangioma tissue transplantation. A total of 40 hemangioma mice were divided randomly into propranolol and physiological saline groups. The animals received propranolol and phys- iological saline treatment separately. Five mice in each group were sacrificed randomly every 7 days. Tumor volume was measured. CD133 and Ki-67 in cells were tested by immunohistochemistry. And CD133 and CD31 in tumor were detected by reverse transcription-polymerase chain reaction （RT- PCR）. Results After 14 days, tumor volume in propranolol group was significantly smaller than that in physiological saline group （70. 6 ± 4. 5 vs. 93.4 ± 6. 3） rnm3 （P〈0. 05）. In propranolol group, the CD133 and Ki-67 positive cell rate of tumor at Day 28 were significantly lower than that at Day 7（35.6 ± 1.8 vs 65.4± 4. 5;8. 8 ± 0. 7 vs 45.4 ± 6. 1） （P〈0. 01）. In propranolol group, the expression of CD133 declined while the expression of CD31 increased at Day 28 （0. 59 ± 0. 05, 0. 20 ± 0. 02 ; 0. 30 ± 0. 03, 1.25 ± 0. 02）. In physiological saline group, both the positive cell rate of CD133, Ki-67 （65.4 ± 5. 1,45. 4 ± 6. 4）and the expression of CD133 and CD31 （0. 59 ± 0. 05, 0. 30 ± 0. 03）showed insignif- icant changes at different timepoints （P〉0. 05）. Conclusions Propranolol has significant therapeutic effects on proliferating hemangioma in nude mice. And the mechanism during prophase is associated with mature endothelial cells derived from vascular endothelial progenitor cells.
出处
《中华小儿外科杂志》
CSCD
北大核心
2014年第2期89-92,共4页
Chinese Journal of Pediatric Surgery
关键词
血管瘤
普萘洛尔
小鼠
裸
Hemangioma
Propranolol
Mice,nude
