摘要
目的 探讨脂蛋白酯酶（LPL） HindⅢ基因多态性与脑出血的相关性.方法 采用病例对照研究方法、PCR-RFLP方法以及基因扩增产物测序方法,检测120例脑出血患者（脑出血组）和147例健康体检者（对照组）LPLHindⅢ基因多态性,并测定血脂和血糖.结果 脑出血组T和G等位基因频率分别为90.8％和9.2％;对照组分别为82.3％和17.7％.脑出血组G等住基因频率和GG基因型检出率明显低于对照组,TG、LDL-C、空腹血糖、收缩压、舒张压明显高于对照组（P＜0.05,P＜0.01）.TT基因型人群TG水平较TG＋ GG基因型人群明显升高（P＜0.05）.调整年龄、高血压、高血糖等相关因素,应用多元logistic回归分析显示,LPL HindⅢG等位基因可能是一个保护性因素（OR=0.392,95％CI：0.191～0.805,P=0.011）.结论 LPL HindⅢ基因多态性与脑出血有一定关联,LPL HindⅢG等位基因突变可能是脑出血发病的一个保护因素.
Objective To study the correlation between polymorphism of lipoprotein lipase （LPL）HindⅢ gene and cerebral hemorrhage.Methods One hundred and twenty cerebral hemorrhage patients served as a cerebral hemorrhage group and 147 healthy subjects served as a control group in this study.Polymorphonism of LPL HindⅢ gene was detected in the two groups.Their blood lipid and blood glucose levels were measured.Results The detection rate of T and G allelic gene was 90.8％ and 9.2％ in cerebral hemorrhage group and 82.3％ and 17.7％ in control group.The detection rate of G allelic gene and GG genotype was significantly lower whereas the serum levels of TG,LDL-C,fasting blood glucose,and the systolic and diastolic pressure were significantly higher in cerebral hemorrhage group than in control group （P＜0.05,P＜0.01）.The serum TG level was significantly higher in patients carrying the TT genotype than in those carrying the GG＋TG genotype （P＜0.05）.Multivariate logistic regression analysis showed that the Hind Ⅲ gene polymorphonism was related with cerebral hemorrhage after adjustment for age,hypertension and hyperglycemia （OR=0.392,95％CI：0.191-0.805,P=0.011）.Conclusion LPL Hind Ⅲ gene polymorphonism is related with cerebral hemorrhage.The LPL Hindm G allelic gene mutation may be a protective factor for cerebral hemorrhage.
出处
《中华老年心脑血管病杂志》
CAS
北大核心
2014年第2期129-132,共4页
Chinese Journal of Geriatric Heart,Brain and Vessel Diseases
基金
上海市松江区卫生局科研课题(2012-Ⅲ-13)
关键词
脑出血
脂蛋白脂酶
聚合酶链反应
基因频率
基因型
危险因素
cerebral hemorrhage
lipoprotein lipase
polymerase chain reaction
gene frequency
genotype
risk factors
