摘要
目的研究人羊水干细胞(hAFSC)移植对CCl4诱导性肝硬化大鼠的改善。方法使用贴壁法分离培养羊水干细胞,Western blot法鉴定。Wistar大鼠24只随机分为对照组(n=8)和肝硬化组(n=16),肝硬化组采用60%的CCl4植物油皮下注射7周,制造肝硬化模型,再随机分成肝硬化模型对照组(注射等体积PBS,n=8)、hAFSC移植组(n=8)。直接经肝内注射hAFSC,3周后处死所有大鼠,肝组织石蜡切片进行病理学分析。结果分离的羊水干细胞均表达特异性标记物Oct-4、SSEA-4。与大鼠肝硬化模型组比较。肝硬化程度明显减轻,假小叶附近脂肪化细胞和肝组织中胶原纤维的量明显减少。结论 hAFSC肝内移植可减轻大鼠肝硬化病变程度。
【Objective】To study the effect of the human amniotic fluid stem cell（hAFSC） transplantation on liver cirrhosis induced by carbon tetrachloride in mice.【Methods】Using adherent method,isolate and culture human amniotic fluid stem cells,Western blot identified hAFSC.Experimental Wistar rat（n=24）were randomLy divided into control group（n=8） and liver cirrhosis group（n=16）,which induced by injection of 60% carbon tetrachloride and plant oil complex for 7 weeks,at 3 weels after mouse were challenged with CCl4,liver cirrhosis group were randomLy divided into model group（ouly infuse saline,n=8）,hAFSC transplantation group（n=8）,which hAFSC is direct intrahepatic injected,3 weeks after post-transplantion,all rats were sacrificed,liver tissue paraffin is histological analysis.【Resluts】 The hAFSC transplantation group liver cirrhosis is ameliorated significantly,fat cell near false lobule and liver tissue collagen fibers decreased markedly.【Conclusion】 hAFSC transplantation can ameliorate liver cirrhosis degree of rat model.
出处
《中国医学工程》
2013年第10期1-2,4,共3页
China Medical Engineering
基金
广东省自然科学基金资助(7151202602000007)
关键词
人羊水干细胞
移植
肝硬化
CCL4
human amniotic fluid cell
transplantation
liver cirrhosis
carbon tetrachloride
