摘要
为了促进木霉免疫诱导剂类生物肥料及土壤改良剂在木本植物生长中的应用,采用盆栽试验方法,以T1(5×102 cfu·cm-3)、T2(5×103 cfu·cm-3)和T3(5×104 cfu·cm-3)3个水平的木霉分生孢子诱导杨树苗60d,研究根施棘孢木霉ACCC30536分生孢子悬液后山新杨的生长量及土壤中水解氮含量变化规律。结果表明:T2水平诱导的效果最佳,杨树苗在15d内的株高和茎基生长量的最大值分别为7.01cm和0.15cm,是对照CON4(不施木霉)的5.12和2.14倍;单株杨树苗根的平均干重为7.38g,较CON4增加50.92%;单株杨树苗茎的平均干重为4.24g,较CON4、T1和T3分别增加了16.48%、49.82%和7.34%。同时,以T2水平木霉诱导杨树苗30d,种植杨树苗的盆土中水解氮含量较CON(不施用木霉且不栽培杨树苗的盆土)、CON2(施用T2水平的木霉但不栽培杨树苗的盆土)及CON4分别提高16.81%、9.5%和20.26%。说明棘孢木霉具有极显著促进杨树苗生长和改善土壤中水解氮含量的作用,最佳诱导量为5×103 cfu·cm-3。
In order to improve the application of T richoderma asperellum immune-inducer-like bio-fertilizer and soil amendment in benefiting growth of woody plants ,pot culturing experiment was carried out to study the effects of T richoderma asperellum ACCC30536 spore suspension of different concentrations （T1 ,5 × 102 cfu·cm-3 ;T2 ,5×103 cfu·cm-3and T3 ,5×104 cfu·cm-3）on Populus davidiana× P.bolleanagrowth ,the spore suspensions were added to its root to induce the growth for 60 days ,the plants and available nitrogen content in soil were measured .The results showed that T2 induction had significant efficiency ,the seedlings achieved maximum plant height and stem base perimeter increased by 7 .01 cm and 0 .15 cm respectively for 15 days , which were 5 .12 and 2 .14 times more than control CON4（without Trichoderma asperellum treatment） .The average dry root weight of T 2 seedlings was 7 .38 g ,which increased by 50 .92% compared with CON4 ,and av-erage dry stem weight of T2 seedlings was 4 .24 g ,which increased by 16 .48% ,49 .82% and 7 .34% compared with CON4 ,T1 and T3 respectively .Simultaneously ,after inducing for 30 days ,the available nitrogen content of T2 pot soil increased by 16 .81% compared with CON1（pot soil with neither seedling nor inducing ） ,9 .5%compared with CON2（pot soil with inducing as T2 without seedling）and 20 .26% compared with CON4 .The results above showed that T .asperellum had significant effects on the improvement of growth for Shanxinyang and the available nitrogen content of the pot soil ,the best concentration of inducing spore suspention was 5 × 103 cf u · cm -3 .
出处
《黑龙江农业科学》
2014年第2期45-49,共5页
Heilongjiang Agricultural Sciences
基金
东北林业大学本科生创新训练资助项目(2012 10225099)
黑龙江省自然科学基金资助项目(C201216)
国家自然科学基金资助项目(31170601)
关键词
棘孢木霉
山新杨
生长量
水解氮含量
Trichoderma asperellum
Populus davidiana ＆#215
P .bolleana
growth
available nitrogen content
