摘要
以草木樨状黄芪无菌苗茎切段为材料 ,在含 1～ 2 mg/L 2 ,4- D和 0～ 0 .5mg/L 6BA的 MS培养基上培养获得大量愈伤组织。愈伤组织诱导率在 95%以上。愈伤组织在附加 0 .2mg/L KT,1 mg/L6BA,0或 0 .5mg/L NAA,50 0 mg/L CH和 2 0 0 m/L YE的 MS培养基上诱导丛生芽 ,并进而发育成苗。苗的分化频率为 1 0 0 %。分化苗或其茎的切段在不加任何外源植物激素的 1 /2 MS培养基上即可出现根的分化 ,分化频率达 90 %以上。再生植株经炼苗后移栽成活率达 80 %以上。
Calluses were induced form internode segments of sterile seedlings of Astragalus melilotoides Pall on MS medium supplemented with 1-2 mg/L 2,4 D and 0-0.5 mg/L 6BA.The frequency of callus induction was above 95%.When calluses were transferred and cultured on MS medium supplemented with 0.2 mg/L KT,1 mg/L 6BA,0 or 0.5 mg/L NAA,500 mg/L CH and 200 mg/L YE,numerous shoots were formed.The differentiation frequency was 100%.The regenerated shoots or shoot cuttings produced roots on 1/2 MS without any phytohormones.The regenerated plantlets were transplanted into the soil.Over 80% transplanted plants were survival.
出处
《西北植物学报》
CAS
CSCD
2001年第1期136-141,T005,共7页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金资助项目(39770365)
关键词
草木樨状黄芪
离体培养
植株再生
Astragalus melilotoides Pall
in vitro culture
plant regeneration
