摘要
运用加速溶剂萃取技术(accelerated solvent extraction,ASE),通过单因素试验和响应面分析法优化海刀豆(Canavalia maritima)中总黄酮的提取工艺。在单因素试验的基础上,选取萃取温度、萃取时间和甲醇体积分数3个因素为自变量,总黄酮提取率为响应值,采用Box-Behnken试验设计方法,研究各因素的显著性及其交互作用对总黄酮提取率的影响。利用Design Expert软件,建立了提取率与各因素的二次多项式数学模型,得到了海刀豆中总黄酮的最佳提取条件:萃取压力10.3 MPa,萃取温度130℃,萃取时间16 min,甲醇体积分数66%,循环次数2次,总黄酮提取率为83.43%,与理论值相对偏差-1.63%。
Based on a single-factor analysis, we used Response Surface Methodology （RSM） to optimize the method for extraction of flavonoids by accelerated solvent extraction（ASE）technology from Canavalia maritima. The extraction temperature,static extraction time and methanol concentration were chosen as independent variables and the flavonoids extraction rate was selected as response value; Box-Behnken method was adopted for the designment of the experiment to study the significance of each factor and its mutual influence on extraction rate. Then we utilized Design Expert software to establish the mathematical model of polynomial quadratic equation. The optimum conditions of flavonoids extraction were as follows： 130 ℃ of extraction temperature, 16 min of extraction time, 66% of methanol concentration and static circulate twice. The extraction rate was 83.43% at the optimum conditions and the relative error between the actual and predicted values was only 1.63%.
出处
《南方水产科学》
CAS
CSCD
北大核心
2014年第1期78-85,共8页
South China Fisheries Science
基金
国家自然科学基金项目(21205138)
公益性行业(农业)科研专项经费项目(200903030)
海洋公益性行业科研专项(201005020-7)
关键词
海刀豆
黄酮
加速溶剂萃取
响应面分析
Canavalia maritima
flavonoids
accelerated solvent extraction
response surface methodology
