摘要
目的:观察中药乳康饮对移植瘤小鼠乳腺癌血管内皮生长因子C/D(VEGF-C/D)、血管内皮生长因子受体(VEGFR-3)mRNA表达的影响,探讨其抑制乳腺癌转移的机制。方法:30只BALB/c裸鼠乳腺原位种植人乳腺癌细胞株MDA-MB-435S,建立乳腺癌自发性转移模型,随机分为模型对照组,5-FU对照组,乳康饮小、中、大剂量组,乳康饮+5-FU组,每组5只。模型对照组给予0.9%氯化钠溶液0.4mL/d灌胃;5-FU对照组给予5-FU 30mg·kg-1·d-1腹腔注射;乳康饮小、中、大剂量组分别给予乳康饮18、45、90g·kg-1·d-1灌胃;乳康饮+5-FU组给予5-FU注射液30mg·kg-1·d-1腹腔注射,同时给予乳康饮45g·kg-1·d-1灌胃,均每天1次,连续6周。用药后检测肿瘤质量和腋窝淋巴结转移抑制率,肿瘤组织VEGF-C/D、VEGFR-3基因的表达。结果:乳康饮各剂量组及乳康饮+5-FU组肿瘤质量与模型对照组比较明显减小(P<0.01),乳康饮+5-FU组抑瘤率及腋窝淋巴结转移抑制率明显高于其他药物组(P<0.05)。乳康饮各剂量组VEGF-D mRNA表达显著下调,与模型对照组比较,差异均有统计学意义(P<0.05,P<0.01)。乳康饮大剂量组和乳康饮+5-FU组VEGF-C mRNA、VEGFR-3 mRNA表达量下调,与模型组比较,差异有统计学意义(P<0.05,P<0.01)。结论:乳康饮可能通过干预VEGF-C/D、VEGFR-3 mRNA的表达,影响淋巴管的生成,抑制乳腺癌淋巴转移。
Objective： To observe the influence of traditional Chinese medicine compound Rukangyin （RKY） on expressions of VEGF-C/D, VEGFR-3 mRNA in transplantation tumor of mice breast cancer, and to discuss the inhibition mechanism of breast cancer metastasis. Methods： Human breast cancer cell line MDA-MB-435S were in situ implanted into the mammary fat pad of 30 female nude mice to establish breast cancer transplantation tumor spontaneous metastasis models. They were randomly divided into six groups, the model control group, the 5-FU control group, the small, medium, large dose RKY groups and the medium dose RKY＋5-FU group, 5 mice in each group. Normal saline was given to mice in the model control group at the daily dose of 0.4mL by gastrogavage. 5-FU was given to mice in the 5-FU control group at the daily dose of 30mg/kg by peritoneal injection. RKY was given to mice in the small, medium, large dose RKY groups at the dally dose of 18, 45 and 90g/kg by gastrogavage, respectively. 5-FU 30mg·kg-1·d-1 （by peritoneal injection）＋RKY 45g·kg-1·d-1（by gastrogavage） were given to mice in the medium dose RKY＋5-FU group. All medications were carried out once dally for 6 successive weeks. The tumor volume, the tumor inhibition ratio and the inhibition ratio of axillary lymph node metastasis were detected after medications treatment. The expression of VEGF-C/D and VEGFR-3 of the breast cancer tissues were detected using immunohistochemical assay. Results： Compared with the model control group, the tumor volume was markedly reduced in the small, medium, large dose RKY groups and the medium dose RKY＋5-FU group （P〈0.01）. The inhibition rate of tumor and axillary lymph node metastasis were increased （P〈0.05）. The inhibition rate of tumor and axillary lymph node metastasis were highest in the medium dose RKY＋5-FU group. Compared with the model control group, the expressions of VEGF-C/D and VEGFR-3 were reduced significantly in each dose RKY groups. The difference was statistically significant �
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2014年第2期594-597,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
山东省自然科学基金(No.ZR2009CM039)
山东省医药卫生科技发展计划(No.2011HW084)~~
关键词
乳腺癌
乳康饮
淋巴管生成
血管内皮生长因子C
D
血管内皮生长因子受体-3
Breast cancer
Rukangyin
Lymphangiogenesis
Vascular endothelial growth factor C/D
Vascular endothelial growth factor receptor 3
