摘要
目的 :探讨p53基因突变与大肠癌临床病理特点的关系。方法 :应用PCR SSCP技术检测 135例大肠癌患者p53基因第 5、6、7、8外显子的突变。结果 :153例大肠癌患者p53基因的突变率为54 0 7% ,p53基因突变与大肠癌的病理分级、临床分期、淋巴结转移及年龄均有关。结论 :p53基因与大肠癌的发生、发展及转归均有明显的关系。检测p53基因突变既可作为大肠癌的诊断方法 ,也可作为大肠癌预后的判定指标。
Objective:To study the reationship between mutation of p53 gene and clinicopathology in colorectal cancer.Methods:The mutations of exon 5,6,7 and 8 of p53 gene in 135 patient with colcorectal cancer were detected by PCR SSCP.Results:The mutation rate of p53 gene was 54.07%,and the mutation of p53 gene was correlated with histologie grade,clinical stage,metastasis of lymph nodes and patients'age.Conclusions:p53 genemay play an important role in the onset,development and outcome of colorectal cancer,and detection of mutation of p53 might be a diagnotic method as well as a prognostic factor in colorectal cancer.
出处
《肿瘤防治杂志》
2000年第6期600-603,共4页
China Journal of Cancer Prevention and Treatment
