摘要
以中华红叶杨茎段为外植体，研究了温度及光照条件对其褐变的影响。结果表明：外植体低温预处理与简单培养基培养相结合，抑制褐化效果好；培养初期进行暗培养能有效降低褐化率，以暗培养10d为宜；温度对褐化率也有影响，在一定范围内，温度越高，褐化越严重，低温可较好地减轻褐化；光照强度1000－3000lx对褐化率的影响不大。
Taking the stem of Populus × euramericana cv. Zhonghuahongye as explant, the effect of temperature and light condition on explants browning of it were studied. The results showed that low temperature pretreatment combined with simple culture medium culture could decrease browning rate and had a good browning decreasing effect; dark culture decreased browning rate effectively at the first stage and ten days was an optimum; temperature also affected browning rate, within a certain range the higher the temperature was, the more serious the browning was, and low temperature lightened browningj the light intensity in 1 000-3 000 lx could not cause an obvious effect for the browning rate.
出处
《北方园艺》
CAS
北大核心
2014年第4期94-96,共3页
Northern Horticulture
关键词
中华红叶杨
温度
光照
褐变
影响
Populus × euramericana cv. Zhonghuahongyei temperature
light
browning
effect
