摘要
以地被菊‘矮黄’、‘玉龙’和传统菊花‘金背大红’的叶盘和茎段为外植体进行再生培养 .实验结果表明 ,不同品种对相同激素水平的反应是不同的 :传统的大菊‘金背大红’的愈伤诱导率和分化率都远远低于两种地被菊 ;在相同条件下以茎段为外植体诱导愈伤率高于叶盘 ,但是叶盘的直接分化率高于茎段 .‘玉龙’和‘矮黄’的最适再生培养基组成为MS +NAA 0 .1mg/L + 6 BA 1mg/L和MS +NAA 0 .2mg/L + 6 BA 2mg/L ,‘金背大红’为MS +NAA 1mg/L + 6 BA
Adventitious shoots were regenerated from leaf and stem explants of three chrysanthemum cultivars. The optimum medium for both explant types in ‘Aihuang’ and ‘Yulong’ containd Murashige & Skoog basal medium supplemented with NAA 0.1?mg/L+6\|BA 1?mg/L and NAA 0.2?mg/L+6\|BA 2?mg/L, in ‘Jinbei Dahong’ contained Murashige & Skoog basal medium supplemented with NAA 1?mg/L+6\|BA 5?mg/L. Generally, stem explants were superior to leaf explants. There were large cultivar difference in shoot regeneration frequency with three cultivars failing to respond over a wide range of hormone combination.
出处
《北京林业大学学报》
CAS
CSCD
北大核心
2001年第1期32-33,共2页
Journal of Beijing Forestry University
基金
国家林业局"948"花卉高产栽培及花期调控技术项目资助! (9840 )
关键词
菊花
外植体
再生体系
茎段叶盘
Dendrathema grandiflorum, explants, shoot regeneration
