摘要
为了提高 Ｂ．ｔ． ＭＰ— ３４２菌株对甜菜夜蛾的毒力．以 Ｂ．ｔ． ＭＰ— ３４２菌株作亲株．根据 ＭＰ—３４２菌株基因和毒蛋白的组成、采用重组ＤＮＡ技术将对甜菜菜夜蛾高毒的ＣｒｙｌＣａ基因引入ＭＰ—３４２菌株。构建了工程菌Ｂ．t．ＭＰ－３４２ＩＣ－１５工程菌。对该菌检测结果表明：ＭＰ－３４２ＩＣ－１５工程菌表达两种Ｃryl类晶体蛋白．其分子量分别为 １３５ｋＤ（ＣｒｙｌＣａ）和 １３３ｋＤ（Ｃｒｙ１Ａｃ）。其Ｃｒｙ１Ｃａ基因蛋白表达量占 Ｃｒｙｌ类晶体蛋白的 ３５％和总晶体蛋白的 ２９％． 工程菌能良好表达ＣｒｙｌＣａ基因蛋白．且比例合适。据工程菌 Ｂ．ｔ． ＭＰ—３４２ＩＣ— １５对甜菜夜蛾的生测表明．其毒力较亲株 ＭＰ－０ ３４２提高近 １倍。对工程菌和亲株发酵上清液增效日子的增效活性进行了测定： 工程菌上清液增效因子对小菜蛾具有一定增效作用；亲株上清液未显示对小菜蛾的增效作用。
B. t. MP - 342 strain was transformed with the gene of Cry1Ca which was toxic to Spodopteraexigua. and the genetically engineered microorganism of B. t. MP - 342IC - 15 was constructed. MP -342IC -15 expressed 135kD (CrylCa) and 133kD (Cry1Ac) insecticide crystal protein. The percentage of CrylCa protein in this recombinant was 35% of Cryl proteins and 29% of total crystal proteins. LC 50 of MP - 342IC- 15 to neonate of S. exigua was one fold lower than that of parent strain, The supernatant potentiator of MP - 342IC - 15 played a fixed role in the death of Pluttella xylostella but the supernatant of parent strain did not.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2001年第1期133-137,共5页
Journal of Northeast Forestry University
基金
内蒙古自治区"九五’重点科技项目
