摘要
目的 探讨血必净与乌司他丁对内毒素（LPS）致绵羊高原多器官功能障碍综合征（H-MODS）的防治作用.方法 将25只绵羊从亚高原地区兰州（海拔1500 m）运输到高原地区桑科草原（海拔3200 m）后随机分为对照组（A组）、模型组（B组）、乌司他丁治疗组（C组）、血必净治疗组（D组）.动物全麻后,以6 μg/kg LPS于30 min内静脉泵入建立H-MODS模型.在静脉泵入LPS后,C组、D组分别静脉滴注乌司他丁（30 000 U/kg）、血必净（2 mL/kg）注射液,均每天2次,连续3 d.A组和B组静脉滴注同剂量的生理盐水.于0、6、12、24、48和72 h测定不同时间点各组血清生化指标、血常规.结果 与B组比较,C组和D组动脉血氧饱和度下降幅度较小;治疗组AST、ALT、TBIL和Cr升高低于B组（P〈0.05）;D组在个别时间点各项指标改善优于C组.B、C和D组绵羊的死亡率分别为71.4%、42.8%和28.6%.结论 乌司他丁和血必净均能降低 LPS诱导的H-MODS死亡率,对肺脏、肝脏和肾脏等多器官损伤具有一定的治疗作用,两者比较血必净治疗效果可能优于乌司他丁.
Objective To determine the therapeutic effect of utinastatin （UTI） and Xuebijing （XBJ） on multiple organ dysfunction syndrome at high altitude （H - MODS） in sheep model induced by lipopolysaccharide （LPS）. Methods After 25 sheep were transported from LANZHOU at 1500 m altitude to SANKE grassland at 3200 m altitude, then randomly divided into 4 groups： normal control group （group A）, H -MODS group （group B）, UTI treatment group （group C）, XBJ treatment group （group D）. The anaesthetized sheep received intravenous infusion of LPS （6 Ixg/kg） to induced H - MODS in group B, group C and group D. The animals received injection of UTI （30 000 U/kg） for 3 days, twice daily in group C and XBJ （2 mL/kg）for 3 days twice daily in group D. Blood samples were taken for determination of serum alanine aminotransferase （ ALT）, aspartate aminotransferase （ AST）, creatinine （Cr）, total bilirubin（TBIL） and blood routine at O（basement）, 6, 12, 24, 48 and 72 h respectively. Results PaO2 decreased as early as at 6 h after injection of LPS. Compared with group B, PaO2 decreased less significantly in two treatment groups. AST, ALT, TBIL and Cr were significantly lower than those in group B （ P 〈 0.05）, also there were obvious differences at some times between group C and group D. The animal mortality was 71.4%, 42.8% and 28.6% in group B, group C and group D respectively. Conclusion Application of UTI and XBJ could reduce the mortality of H - MODS animals induced by LPS. They have protective effects on lung, liver and kidney injuries in H - MODS animals. XBJ would have an advantage over UTI in protective effect.
出处
《中国急救医学》
CAS
CSCD
北大核心
2014年第2期175-178,共4页
Chinese Journal of Critical Care Medicine
基金
甘肃省自然科学基金项目（1010RJZA052）
