摘要
目的建立加味藿香正气丸中厚朴酚与和厚朴酚含量测定的高效液相色谱(HPLC)分析方法。方法采用Agilent ZORBAX SB-C18(4.6 mm×150.0 mm,5μm)色谱柱,流动相为甲醇-水(70∶30),检测波长为294 nm,流速为1.0 mL/min,进样量10μL,柱温25℃。结果厚朴酚与和厚朴酚的线性范围分别为0.113 0~1.130 0μg[相关系数(r)=0.999 99,n=6]和0.061 5~0.615 0μg(r=0.999 98,n=6);平均回收率分别为98.4%[相对标准偏差(RSD)=0.98%,n=9]和98.8%(RSD=0.54%,n=9)。结论 HPLC法测定加味藿香正气丸中厚朴酚与和厚朴酚的含量,操作简便、准确、重现性好,可作为该药品质量控制的方法。
Objective To establish an analytic method of high performance liquid chromatography （HPLC） for determi- nation of Magnolol and Honokiol in Jiaweihuoxiangzhengqi pills. Methods The chromatographic column of Agilent ZORBAX SB-Cls（4.6 mmxl50.0 mm,5 p,m）was adopted,and the mobile phase consisted of methanol-water（70：30） with the detection wave- length of 294 nm,flow velocity of 1.0 mL/min, sample size of 10 p,L and column temperature of 25 ~C. Results The hnear range of Magnolol and Honokiol was 0.113 0-1.130 0 I^g [correlation coefficient （r） =0.999 99, n=6）and 0.061 5-0.615 0 p,g （r=-0.999 98, n=6） respectively;the average recovery rate of Magnolol and Honokiol was 98.4% [relative standard deviation （RSD）=0.98%, n=9） and 98.8%（RSD=0.54% ,n=9）. Conclusion HPLC for determination of Magnolol and Honokiol in Jiaweihuoxiangzhengqi pills is simple, accurate and highly reproductive, it can be used for the quality control of this preparation.
出处
《现代医药卫生》
2014年第4期499-500,502,共3页
Journal of Modern Medicine & Health
关键词
厚朴酚
和厚朴酚
色谱法
高压液相
含量测定
加味藿香正气丸
Magnolol
Honokiol
Chromatography,highpressureliquid
Determination
Jiaweihuoxiangzhengqipills
