摘要
目的：探讨睾酮对睾丸支持细胞TM4氧化应激损伤和细胞凋亡的影响。方法在支持细胞系TM4细胞培养液内分别加入H2O2和睾酮，分为空白组、模型组、低、高剂量睾酮组。在作用24 h后，用MTT法检测吸光度值，用硫代巴比妥酸比色法、黄嘌呤氧化酶法测定SOD活性及MDA含量，用流式细胞仪测定细胞凋亡率和存活率，用实时PCR检测caspase-3 mRNA的表达。结果睾酮低、高剂量组的吸光值由模型组的（0.33±0.05）分别增加至（0.42±0.05）、（0.46±0.07）；SOD活性由（9.01±0.48）U/ml分别增加至（11.02±1.15）U/ml、（12.51±0.98）U/ml；MDA含量由（6.41±0.62）mol/L分别降低至（5.37±0.47）mol/L、（4.28±0.44）mol/L；细胞的凋亡率由（20.10±1.32）%分别降低至（16.13±0.49）%、（10.63±2.29）%；存活率由（68.40±1.00）%分别增加至（73.63±2.94）、（76.87±6.58）；caspase-3 mRNA的表达量由（2.85±0.09）分别降低为（2.34±0.27）、（1.85±0.11）。结论睾酮能改善睾丸支持细胞的氧化应激损伤，抑制细胞凋亡。
Objective To investigate the effect of testosterone on oxidative stress and apoptosis of TM4 Sertoli cells. Methods H2O2 and testosterone were added into medium to stimulate TM4 cells. Cells were divided in the control group, the model group, low and high dose of testosterone group. After 24 h stimulation, proliferation of the cells were detected by MTT, the SOD activity and MDA content in cells were measured by thiobarbituric acid and xanthine oxidase method respectively, the apoptosis and survival rates of cells were determined by flow cytometry, the caspase-3 mRNA expression by real time-PCR. Results The absorbance value of TM4 cells increased from 0.33±0.05 of the model group to 0.42±0.05, 0.46 ±0.07 of the low and high dose of testosterone group. The SOD activity increased from 9.01±0.48 U/ml to 11.02±1.15 U/ml, 12.51±0.98 U/ml respectively. The MDA content decreased from 6.41±0.62 mol/L to 5.37±0.47 mol/L, 4.28±0.44 mol/L respectively. The rate of apoptosis decreased from 20.10±1.32%to 16.13±0.49%, 10.63±2.29%respectively. The rate of cell survival increased from 68.40±1.00%to 73.63±2.94%, 76.87±6.58%respectively. The expression of caspase-3 mRNA decreased from 2.85±0.09 to 2.34±0.27, 1.85±0.11 respectively. Conclusion Testosterone may improve the cells injuried by oxidative stress and inhibit the apoptosis of TM4 cells.
出处
《中国男科学杂志》
CAS
CSCD
北大核心
2013年第11期8-11,共4页
Chinese Journal of Andrology
