摘要
目的应用荧光原位杂交技术(fluorescence in situ hybridization,FISH)检测分析3、7、17号染色体及9p21位点异常与尿路上皮细胞癌发生、发展的关系,评估其用于尿路上皮细胞癌诊断的可行性和临床应用前景。方法选取2011年7月至2013年8月在青海红十字医院泌尿外科经膀胱镜活检确诊为尿路上皮细胞癌的患者116例作为实验组,另外选取正常体检者92例作为对照组,分别行尿脱落细胞学检查和FISH检测,比较两种方法诊断的特异性和灵敏性,统计分析3、7和17号染色体及9p21位点的畸变率与尿路上皮肿瘤分级的相关性。结果①尿路上皮细胞癌患者中,FISH和细胞学诊断尿路上皮细胞癌的灵敏度分别为81.0%和37.9%;对照组中,FISH和细胞学诊断尿路上皮细胞癌的特异度分别为90.2%和93.4%。②各染色体及区带呈较高的畸变发生率,3、7、l7号染色体畸变阳性率分别为94.3%、95.6%和88.5%;9号染色体p21位点畸变阳性率为63.2%。③FISH的灵敏性随着肿瘤分级分期的升高而增高。结论 FISH技术能明显改进尿路上皮细胞癌的诊断,对于尿路上皮细胞癌的诊断及复发监测具有重要意义。
Objective To evaluate the feasible and clinical usefulness prospect of fluorescence in si- tu hybridization （FISH） testing the genetic mutations on chromosome 3,7,17 and 9p21 and analyzing rela- L tionship with origins and development of urothelial cell carcinomas. Methods 116 patients admitted in our hospital during July 2011 through August 2013 who were diagnosed with urothelial cell carcino- ma by cystoscopy and biopsy were included in this study as study group. Meanwhile, 92 check-up patients were included as a control group. Urine cytology and urine FISH were tested on the patients in both groups. Sensitivity and specificity of above the two methods were compared. The relevance of the mutation rates of chromosome and tumor pathology grades were analyzed as well. Results ①The sensitivity and the specificity of urine FISH and urine cytology in the patients with urothelial cancer 81.0% and 37.9% ,90.2% and 93.4% relatively. ② The genetic mutations on chromosome in the patients with urothelial cell carcinoma presented a significant higher occurring rates, which were 94.3% ,95.6% and 88.5% on chromosome 3,7 and 17 relatively, and 63.2% on chromosome 9p21. ③ The sensitivity of urine FISH was raised with tumor pathology grade. Conclusions Urine FISH testing can improved the clinical diagnosis and surveillance of urothelial cell carcinoma , com- pared to routine urine cytology.
出处
《现代泌尿生殖肿瘤杂志》
2013年第6期353-355,共3页
Journal of Contemporary Urologic and Reproductive Oncology
关键词
荧光原位杂交技术
尿路上皮细胞癌
诊断
Fluorescence in situ hybridization （FISH）
Urothelial cell carcinoma
Diagnosis
