摘要
为深入研究黄连解毒汤体内发挥疗效的效应物质基础,本文采用正负离子扫描模式下的LC-FT-ICR-MS方法,通过与黄连解毒汤提取物和混合对照品的保留时间和高分辨质谱信号比较分析,发现并确证了给予黄连解毒汤后大鼠血浆中主要的38个入血成分,包括22个原形成分和16个代谢产物。在原型成分中,16个原型化合物通过与对照品比对得到了准确鉴定;在代谢产物中,除M11为一相代谢产物外,黄连解毒汤主要成分在大鼠体内多以二相代谢产物形式存在,包括葡糖醛酸和硫酸化产物。该研究通过对黄连解毒汤入血成分的分析,基本阐明了黄连解毒汤体内发挥药效的可能作用物质基础,为进一步的药理作用及其机制研究提供了依据。
To obtain chemical constituent information of rat plasma after oral administration of Huang-Lian- Jie-Du Decoction （HLJDD）, a LC-FT-ICR-MS method has been established, and both positive and negative ions scan modes were include in the analysis. By comparing their retention time, high resolution mass data of HLJDD extracts, blank plasma and dosed plasma, 38 constituents, including 22 prototype compounds and 16 metabolites, were detected in rat plasma after oral administration of HLJDD. In the 22 prototype compounds, 16 constituents were determined unambiguously by comparing with references. In the analysis of metabolites, phase II reactions like glucuronidation and sulfation were the major biotransformation pathways of HLJDD. M11 was observed as the only phase I metabolite in present experiment. The results will be beneficial for the further pharmacokinetics and pharmacological evaluations of HLJDD.
出处
《药学学报》
CAS
CSCD
北大核心
2014年第2期237-243,共7页
Acta Pharmaceutica Sinica
基金
国家科技支撑计划项目(2008BAI51B02)
国家自然科学基金资助项目(81202904)
