摘要
目的 观察体外转染微小RNA-126（miR-126）对人肺腺癌A549细胞增殖的影响.方法 人肺腺癌A549细胞株进行体外培养,分为3组：A组用单纯脂质体处理,B组用miR-126对照系列处理,C组用miR-126处理.实时定量聚合酶链反应（Real-time PCR）检测miR-126表达,Western blot检测血管内皮生长因子（VEGF）蛋白的表达,噻唑蓝（MTT）比色法检测A549细胞增殖.结果 转染人工合成的miR-126后的C组miR-126表达上调至（0.786±0.132）,与A组（0.453±0.085）、B组（0.432±0.092）比较差异有统计学意义（P＜0.01）;C组细胞的VEGF表达下调至（0.312±0.075）,与A组（0.633±0.172）、B组（0.597±0.095）比较差异有统计学意义（P＜0.01）;C组的细胞增殖被明显抑制,与对照组比较差异有统计学意义（P＜0.05）.结论 转染miR-126能够明显增加A549细胞内miR-126的表达,并下调VEGF蛋白表达,抑制肺腺癌A549细胞生长.
Objective To explore the effect of microRNA-126 （miR-126） on the proliferation of lung adenocarcinoma A549 cells.Methods 549 cells in vitro were divided into 3 groups：group A treated only with liposomes,group B with miR-126 control,and group C with miR-126.The expression of miR-126 was detected by using Real-time quantitative polymerase chain reaction （Real-time PCR）,and that of vascular endothelial growth factor （VEGF） protein by Western blotting,and methyl thiazol tetrazolium （MTr） assay was used to examine the growth of A549 cells.Results In group C,the expression level of miR-126 was increased to （0.786 ±0.132） as compared with group A （0.453 ±0.085） and group B （0.432 ±0.092） （P 〈0.01）,and the VEGF protein expression was decreased to （0.312 ± 0.075） as compared with group A （0.633 ±0.172） and group B （0.597 ±0.095） （P 〈0.01）.The growth of A549 cells in group C was inhibited significantly as compared with group A and group B （P 〈 0.05）.Conclusion MiR-126 transfection can significantly increase its expression in A549 cells,decrease the expression of VEGF protein,and suppress the cells growth.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2014年第2期341-343,共3页
Chinese Journal of Experimental Surgery
基金
广东省科技计划资助项目（2011B080701100）
关键词
微小RNA-126
血管内皮生长因子
增殖
肺腺癌
MicroRNA-126
Vascular endothelial growth factor
Proliferation
Lung adenocarcinoma
