摘要
目的研究桑黄菌发酵过程中酶活性的变化及其和胞外多糖量的关系。方法在发酵过程中测定原始菌株和变异菌株的羧甲基纤维素酶、滤纸酶、木质素过氧化物酶(LiP)、锰过氧化物酶(MnP)、漆酶(Lac)活性变化及培养基中胞外多糖量变化。结果 2菌株的羧甲基纤维素酶和滤纸酶的活性变化呈单峰曲线,分别在第4天和第6天达到峰值;原始菌株和变异菌株的LiP、锰过氧化物酶和漆酶活性的变化呈双峰曲线,且原始菌株和变异菌株LiP的酶促反应米氏常数(Km)分别为25.96、27.07μg/mL;MnP的Km分别为13.28、13.49 mmol/mL;Lac的Km分别为0.12、0.21 mmol/mL;培养基中多糖量前期快速下降,10 d后趋于稳定。结论桑黄菌发酵时能产生较全面的分解木质素和纤维素的酶系统,原始菌株和变异菌株产生的LiP和MnP为同种酶,Lac为同功酶。桑黄菌胞外酶活性变化和培养基中胞外多糖量变化有一定关系。
Objective To study the changes of enzyme activity and its relationship with the content of extracellular polysaccharide during the fermentative process of Phellinus igniarius. Methods The enzyme activities of carboxymethyl cellulase （CMC）, filter paper enzyme （FPE）, lignin peroxidase （LIP）, manganese peroxidase （MnP）, laccase （Lac）, and medium extracellular polysaccharide content during the fermentative process of the original strains and mutant strains were investigated. Results The results showed that the changes of CMC and FPE activities of two strains showed a single-peak curve, peaks of which appeared in days 4 and 6, respectively, but the changes of LiP, MnP, and Lac activities showed a double-peak curve, and the Michaelis constant Km values of Lip in the original strains and mutant strains were 25.96 and 27.07 ug/mL. At the same time, the Km values of MnP were 13.28 and 13.49 mmol/mL, and Km values of Lac were 0.12 and 0.21 mmol/mL, respectively. Medium extracellular polysaccharide content declined rapidly and then tended stable after 10 d. Conclusion P. igniarius could produce a comprehensive enzyme system which could decompose lignin and cellulose during the fermentative process. LiP and MnP produced by the original strains and mutant strains are the same enzyme respectively, but Lac is isoenzyme. The activity changes of extracellular enzyme in P. igniarius have the relationship with the content of medium extracellular polysaccharide.
出处
《中草药》
CAS
CSCD
北大核心
2014年第3期420-424,共5页
Chinese Traditional and Herbal Drugs
基金
浙江省自然科学基金项目(LQ12C02001)
关键词
桑黄菌
发酵过程
酶活性
羧甲基纤维素酶
滤纸酶
木质素过氧化物酶
锰过氧化物酶
漆酶
Phellinus igniarius （L. ex Fr.） Quel
fermentation process
enzyme activity
carboxymethyl cellulase
filter paper enzyme
lignin peroxidase
manganese peroxidase
laccase
