摘要
目的研究复方阿胶浆(FEJ)对肾性贫血大鼠的治疗作用及其可能的作用机制。方法采用腺嘌呤制备肾性贫血大鼠模型;模型制备成功后,动物随机分为6组:对照组,模型组,FEJ低、中、高剂量(3、6、12 mL/kg)组和促红细胞生成素(EPO,200 U/kg)阳性对照组;连续给药24 d后,检测各组大鼠外周血红细胞(RBC)计数、血红蛋白(Hb)水平、红细胞压积(Hct)和血清中血尿素氮(BUN)、血清肌酐(Scr)、EPO、超氧化物歧化酶(SOD)、还原型谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-Px)的量以及红细胞脆性;采用HE染色法观察肾脏组织病理形态,采用实时定量PCR法检测肾皮质EPO和骨髓单个核细胞EPO受体(EPOR)的mRNA表达水平。结果中、高剂量FEJ均可显著升高外周血RBC、Hb、Hct水平,显著降低BUN、Scr的量,显著升高血清SOD、GSH和GSH-Px的量(P<0.05),同时有升高血清EPO水平的趋势;低、中、高剂量FEJ均可以显著降低红细胞脆性(P<0.05);中、高剂量FEJ能够明显改善肾脏组织病理状态,同时显著上调肾皮质EPO和骨髓单个核细胞EPOR mRNA水平(P<0.05)。结论 FEJ能够在整体上有效治疗肾性贫血大鼠,其发挥治疗作用可能的机制为增强体内自由基清除系统的功能,延缓肾脏病变的进程,降低红细胞脆性;上调肾皮质EPO mRNA和骨髓单个核细胞EPOR mRNA的水平,促进体内EPO的表达,增强骨髓红系造血功能。
Objective To investigate the effects of Fufang E＇jiao Jiang （FEJ） on renal anemia rats and its possible mechanisms. Methods The renal anemia rat model was established using adenine. After the model was successfully established, all rats were randomly divided into six groups： control, model, low-, medium-, and high-dose （3, 6, and 12 mL/kg） FEJ, and erythropoietin （EPO, 200 U/kg） positive control groups. After the successive administration for 24 d, the levels of red blood cell （RBC）, hemoglobin （Hb）, and hematocdt （Hct） in peripheral blood were measured, the blood urea nitrogen （BUN）, serum creatinine （Scr）, EPO, superoxide dismutase （SOD）, reduced glutathione （GSH）, and glutathion peroxidase （GSH-Px） were also analyzed, and the erythrocyte fragility was detected, in each group. The pathologic morphology of kidney tissue was examined by HE staining. The mRNA levels of EPO in renal cortex and EPO receptor （EPOR） in bone marrow mononuclear cells （BMNC） were determined by real-time quantitative PCR. Results Medium- and high-dose FEJ significantly increased the levels of RBC, Hb, and Hot in peripheral blood, significantly reduced the quantities of BUN and Scr, as well as significantly increased the levels of SOD, GSH, and GSH-Px in serum （P 〈 0.05）. Moreover, with the doses of FEJ increasing in three FEJ treatment groups, the level of EPO in serum had a tendency to increase gradually. The erythrocyte fragility was significantly decreased in low-, medium-, and high-dose FEJ groups （P 〈 0.05）. Furthermore, medium- and high-dose FEJ could evidently improve the pathologic morphology of kidney tissue, and up-regulate the mRNA levels of EPO in renal cortex and EPOR in BMNC （P 〈 0.05）. Conclusion FEJ has the therapeutic effects on renal anemia rats on the whole. And the effects are probably attributed to two mechanisms. Firstly, FEJ enhances the function of internal free radical scavenging system, postpones the development of renal lesions, and decrea
出处
《中草药》
CAS
CSCD
北大核心
2014年第3期380-385,共6页
Chinese Traditional and Herbal Drugs
基金
山东省自主创新成果转化重大专项(2010ZHZX1A0415)
关键词
复方阿胶浆
肾性贫血
自由基清除系统
红细胞脆性
造血功能
Fufang E＇jiao Jiang
renal anemia
free radical scavenging system
erythrocyte fragility
hematopoiesis
