摘要
目的建立测定精制红花油中亚油酸含量的高效液相色谱法。方法采用高效液相色谱一紫外法，AgilentSB—C-18柱（250mm×4．6mm，5μm），流动相为乙腈-水（75：25），检测波长为203nm，柱温为35℃，流速为1mL／min。结果亚油酸进样量在0．09～1．8μg范围内与峰面积呈良好线性关系，加样回收率为99．96％，RSD为1．29％（n=6）。结论该方法快速、灵敏、准确，可作为精制红花油的质量控制方法。
Objective To establish a high performance liquid chromatography（HPLC） method of the linoleic acid content in refined re- fined safflower oil. Methods The HPLC- UV method was adopted with the Agilent SB- Ct8 column（250 mm ×4.6 mm, 5 μm） and the mobile phase of acetonitrile-water（75：25）, the flow rate was 1.0 mL/min, the detection wavelength was 203 nm and the column tem- perature was 35 ℃. Results The sample injection size of linoleie acid in the range of 0. 09 - 1.8 μg had the good linear relationship with the peak area, the recovery rate was 99.96%, RSD was 1.29% （n = 6）. Conclusion The method is rapid, sensitive and accurate, which can be used for the quality control method of refined carthamus oil.
出处
《中国药业》
CAS
2014年第3期37-38,共2页
China Pharmaceuticals
关键词
高效液相色谱法
精制红花油
亚油酸
high performance liquid chromatography
refined carthamus oil
linoleic acid
