摘要
目的观察比较不同病理类型肺癌患者和结核性胸膜炎患者胸腔积液中醛缩酶A(ALDOA)的表达水平及其与恶性胸腔积液部分生化指标的相关性。方法收集未经抗炎或类固醇激素治疗的65例肺癌伴恶性胸腔积液患者(恶性组)和15例结核性胸膜炎患者(结核组)胸水。ELISA法检测胸水中ALDOA浓度,化学发光法检测胸水中癌胚抗原(CEA)、乳酸脱氢酶(LDH)含量。结果恶性组ALDOA、CEA、LDH浓度分别为(46.75±21.39)ng/mL、(82.24±56.63)ng/mL和(755.76±382.54)U/L,结核组分别为(23.92±17.21)ng/mL、(2.55±1.67)ng/mL和(388.37±163.87)U/L,恶性组ALDOA、CEA、LDH浓度均明显高于结核组(均P<0.01)。不同病理类型肺癌患者恶性胸腔积液中ALDOA浓度分别为:腺癌(71.65±32.09)ng/mL,小细胞肺癌(22.43±18.23)ng/mL,鳞癌(19.16±13.85)ng/mL,腺癌组明显高于另两组(P<0.05)。CEA浓度分别为:腺癌(112.40±62.71)ng/mL,小细胞肺癌(62.45±54.78)ng/mL,鳞癌(71.87±52.4)ng/mL,腺癌组明显高于另两组(P<0.05)。LDH浓度分别为:腺癌(661.81±328.93)U/L,小细胞肺癌(737.62±315.41)U/L,鳞癌(767.85±503.28)U/L,腺癌组与另两组差异无统计学意义(P>0.05)。恶性组和结核组患者胸水中ALDOA与CEA、LDH均呈正相关关系(P<0.01或0.05)。结论 ALDOA、CEA、LDH表达水平在恶性胸腔积液中明显高于结核性胸腔积液,其中肺腺癌胸腔积液ALDOA、CEA水平明显高于另两种病理类型。不论恶性胸腔积液或结核性胸腔积液,ALDOA均与CEA、LDH水平高度正相关。
Objective To investigated the levels of aldolase A （ALDOA） in pleural effusion in patients with different pathological types of lung cancer and patients with tuberculous pleurisy, and the correlation between ALDOA and carcinoembryonic antigen （CEA） ,lactate dehydrogenase（LDH）. Methods 80 cases of pleural effusion samples were collected,of which 65 cases of lung cancer （ malignant group） and 15 cases of tuberculous pleurisy （TB group）. All the patients were not treated with anti-inflammatory or steroid therapy. ALDOA concentrations in pleural effusion were detected by ELISA and the contents of CEA and LDH in pleural fluid were detected by chemiluminescence assay. Results The levels of ALDOA, CEA and LDH in the malignant group were 46. 75 ±21.39 ng/mL,82. 24±56. 63 ng/mL,755.76 ±382. 54 U/L respectively,and were 23.92 ± 17.21 ng/mL,2. 55 ± 1.67 ng/mL,and 388. 37 ±163. 87 U/L in the TB group respectively. The levels of ALDOA, CEA and LDH in the malignant group were significantly higher than those in the TB group （ P 〈 0. 01 ）. The concentrations of ALDOA in malignant pleural effusion from different pathological types of lung cancer were 71.65 ±32. 09 ng/mL （ adenoearcinoma ）, 22. 43 ±18. 23 ng/mL（ small cell lung cancer）, and 19. 16± 13.85 ng/mL（ squamous cell carcinoma）, respectively. The concentration of ALDOA in malignant pleural effusion from the adenocarcinoma patients was significantly higher than that in the other two types （ P 〈 0. 05 ）. The concentration of CEA was 112. 40±62.71 ng/mL （adenocarcinoma） ,62.45± 54. 78 ng/mL （ small cell lung cancer）, and 71.87± 52.4 ng/mL （ squamous cell carcinoma）, respectively. It was significantly higher in adenocarcinoma than that in other two types （P 〈0.05）. The levels of LDH were 661.81 ± 328.93 U/L （ adenocarcinoma）, 737.62 ± 315.41 U/L （small cell lung cancer） ,767.85 ± 503.28 U/L（ squamous cell carcinoma） , respectively. There was no significant difference in three types（P 〉
出处
《中国呼吸与危重监护杂志》
CAS
2014年第1期63-66,共4页
Chinese Journal of Respiratory and Critical Care Medicine
关键词
酶醛缩酶A
恶性胸腔积液
结核性胸腔积液
肺癌
癌胚抗原
乳酸脱氢酶
Aldolase A
Malignant pleural effusion
Tuberculous pleural effusion
Lungcancer
Carcinoembryonic antigen
Lactate dehydrogenase
