摘要
应用30对COSII引物对刚竹属4个竹种及其一个栽培变型进行PCR扩增,电泳结果显示,所有引物都可以在至少3个竹种中获得特异性PCR产物,共扩增出101条带,其中88条表现出多态性,占87.13%。其中2对引物在5份材料中均扩增出一致的多拷贝条带。利用NTsys 2.10e软件,对5个供试竹种材料的亲缘关系进行了分析,从聚类结果看,当遗传系数为0.658时,5个供试材料可以分为3类,紫竹与花哺鸡竹各自单独成一类,桂竹、罗汉竹和斑竹的亲缘关系比较近,形成第3类。这一分类结果与形态学分类结果大致吻合,表明基于水稻等禾本科植物的基因组序列开发出来的COSII分子标记在竹类植物中具一定通用性,且可用于鉴定竹种间的亲缘关系。
The relationship between 5 bamboo accessions was investigated with 30 single-copy orthologous genes （COSⅡ） markers. All of 30 pairs of primers exhibited specific PCR products in at least 3 out 5 accessions. A total of 101 bands were obtained using COS Ⅱ markers, of which, 88 bands （87.12%） were polymorphic. Several pairs of primers showed multi-copy PCR products in all the 5 bamboo species, but no polymorphisms between the products. Based on polymorphic pattern revealed by the 30 primer pairs, genetic distances were calculated with NTsys 2. 10e software. The 5 samples belonging to Phyllostachys genus were classified into three groups when the coefficient of heredity is 0. 658. Ph. nigra and Ph. glabrata were clustered into two individual groups. While Ph. reticulate, Ph. aurea and Ph. reticulata f. lacrima-deae, with a closer relationship, formed a third group. This classification results based on COSⅡ molecular markers were coincided with the results based on morphological classification. Therefore, this set of COSⅡ molecular markers could be well applied in genetic analysis and other related researches in bamboo.
出处
《核农学报》
CAS
CSCD
北大核心
2013年第12期1797-1802,共6页
Journal of Nuclear Agricultural Sciences
基金
国家自然科学基金(30901155)
973项目(2012CB723008)
浙江省自然科学基金(Y307499)
