摘要
目的 研究乳腺癌腋窝淋巴结微小转移灶对预后的影响。方法 将 4 5例Ⅰ～Ⅱ期常规病理检查腋窝淋巴结转移阴性并随访 10年以上的原发性乳腺癌分成死亡和生存两组 ,以单克隆抗体细胞角蛋白 19为标志物 ,采用“准”连续切片免疫组化染色方法进行淋巴结微转移的回顾性对比研究。结果 死亡组的 2 5例中查出微转移 13例 ( 52 % ) ,生存组的 2 0例无 1例微转移检出 ,两组比较有极显著性差异 (P <0 0 1)。结论 本研究结果说明微转移严重影响病人预后 ,检查淋巴结微小转移灶具有理论与应用价值 ,“准”连续切片方法值得推广。
Abstract To study the prognostic affection of micrometastases in axillary lymph nodes of primary breast cancer.Methods Forty-five cases of stage Ⅰ to Ⅱ primary breast cancer in which axillary lymph nodes were metastatic negative by routine pathological examination were divided into two groups,one is died (25 cases),the other is alive (20 cases) through more than 10 years of follow-up.Sup-serial sections and immunohistochemistry techniques were used to re-examined the whole lymph nodes for micrometastases detection.Monoclonal antibody cytokeratin 19 was used as the primary reagents to identify tumor cells in lymph nodes.Results Immunostaining positive tumor cells were found in 13 cases of died group,while no positive tumor cell was found in alive group ( P <0 01). Conclusion Micrometastasis can affect patient sprognosis significantly.Micrometastases detection in lymph nodes of primary breast cancer is valuable theoretically and practically.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2000年第5期340-342,共3页
Cancer Research on Prevention and Treatment
基金
北京市乳腺癌重点学科发展基金
关键词
乳腺癌
免疫组织化学
淋巴结转移瘤
预后
Breast cancer
Micrometastases
Cytokeratin 19
Immunohistochemistry
