摘要
目的:评价仙丹升血颗粒对原发免疫性血小板减少症模型小鼠的药效学研究。方法:将小鼠随机分为正常对照组、模型组、低剂量组、高剂量组、强的松组,采用免疫法腹腔射外源性抗血清建立原发免疫性血小板减少症小鼠模型,分别运用仙丹升血颗粒、强的松治疗,观察各组小鼠外周血象、巨核细胞、脏器指数及脾脏病理学改变、出凝血试验。结果:仙丹升血颗粒能有效升高造模后小鼠血小板计数(P<0.01);使脾脏巨核细胞数、骨髓巨核细胞数恢复正常(P<0.05);高、低剂量组和强的松治疗组胸腺、肾上腺指数较模型组有明显升高(P<0.05),脾脏指数有明显下降(P<0.01),强的松组和大剂量组巨噬细胞和生发中心的凋亡小体数明显减少(P<0.05);大剂量组和强的松组治疗小鼠出血时间有明显缩短(P<0.01),且出血重量明显减少(P<0.01)。结论:仙丹升血颗粒对原发免疫性血小板减少症模型小鼠有明显治疗作用。
Objective： To assess the pharmacodynamics study of Xiandan Shengxue Granules on primary immune thrombocytopenia in model mice. Methods ： Model mice were randomly divided into normal control group, model group, low dose group,high dose group,and prednisone group. The mice model was established by using the immune method of in- jecting exogenous antiserum into the abdominal cavity. The model mice were respectively treated with Xiandan Shengxue Granules and prednisone. And peripheral blood like megakaryocyte,viscera index, spleen pathological change and the coagulation tests were. Results：Xiandan Shengxue Granules could effectively rise the model mice＇s platelet count （P 〈 0.01 ）, and the number of spleen megakaryocyte and bone marrow megakaryocyte recover were normal （P 〈 0.05 ）. The thymus and adrenal index of high and low dose groups and prednisone group were significantly higher than those of model group （P 〈0.05）, and spleen index was decreased obviously （P 〈 0.01 ）. The number of macrophages and apoptotic body of germinal center of high dose group as well as prednisone group was decreased significantly （ P 〈 0.05 ）. The bleeding time of high dose group and prednisone group was significantly shortened （ P 〈 0.01 ） , and the bleeding weight was decreased significantly （P 〈 0.01 ）. Conclusion ： Xiandan Shengxue Granules has obvious therapeutic effect on the model mice with primary immune thrombocytopenia.
出处
《中华中医药学刊》
CAS
2014年第2期335-337,共3页
Chinese Archives of Traditional Chinese Medicine
基金
四川省中医药管理局项目(2012-G-036)
四川省技术创新项目(2012CD00158)
关键词
仙丹升血颗粒
原发免疫性血小板减少症
药效学研究
Xiandan Shengxue Granules
primary immune thrombocytopeniaenia
pharmacodynamic study
