摘要
目的：研究吉祥草乙酸乙酯部位对人宫颈癌Caski细胞抑制作用及可能的作用机制。方法：用不同浓度的吉祥草乙酸乙酯部位（3．125、4．6875、6．25、9．375、12．5、18．75、25．0、50．0μg／ml）处理宫颈癌Caski细胞24h后，采用MTT法检测不同浓度的吉祥草乙酸乙酯部位在不同作用时间下对Caski细胞增殖率的影响；用12．5、25．0和50．0μg/ml的吉祥草乙酸乙酯部位处JEA．宫颈癌Caski细胞不同作用时间（4、8、12、24、48h）后，MTT法检测其对细胞活力的影响，Hoechst33258染色检测其对细胞凋亡的影响，RT—PCR测定COX-2、BCl-2和BaxmRNA表达，并计算BCl-2与Bax的比值。结果：吉祥草乙酸乙酯部位在3．125—50．0μg/ml浓度范围内对Caski细胞的生长抑制作用呈明显的时间．剂量依赖性，当药物浓度达到50．0μg／ml时，抑制率达到最大到58．4％，并且经IC50分析软件进行计算其IC50值为35．29μg/ml；吉祥草乙酸乙酯部位（12．5、25．0和50．0μg／ml）处理能显著抑制Caski细胞增殖，促进其凋亡（P〈0．05或P〈0．01），当药物浓度为50．0μg/ml，作用时间48h时，其抑制率高达98．9％；上调BaxmRNA表达，下调COX-2、BCl-2mRNA表达和BCI。2与Bax的比值。结论：吉祥草乙酸乙酯部位有显著的抑制Caski细胞增殖和诱导凋亡作用，其作用机制可能与其上调Caski细胞BaxmRNA表达，下调COX-2、BCl-2mRNA表达和BCl-2与Bax的比值有关。
Objective： To investigate the inhibition effects of ethyl acetate extraction from Reineckia carnea （Andr.） Kunth on cervix cancer Caski cells, and to explore the possible mechanism. Methods： Caski cells were treated with different concentration of ethyl acetate extraction from Reineckia eamea （ Andr. ） Kunth （3. 125,4.6875,6.25,9. 375,12.5,18.75,25.0,50.0 lag/ml） for 24h ; After Caski cells were treated with ethyl acetate extraction from Reineekia earnea （Andr.） Ktmth （12.5, 25.0 and 50.0 g/ml） at different times （4, 8, 12, 24, 48h）, cell viability was determined by MrlT assay, the apoptosis cells morphous were observed by hoeehst33258 through fluorescence microscope, the Expressions of COX-2, BC1-2 and Bax mRNA were detected by RT-PCR, and BC1-2/Bax ratio was analyzed. Results： Ethyl acetate extraction from Reineekia carnea （ Andr. ） Kunth from 3. 125 to 50.0 μg/ml concentration range for Caski cells growth inhibition was obvious time-dose dependent, when the concentration of drug at 50.0 μg/ml, reached the maximum inhibition rate to 58.4%, and by the IC50 analysis software to calculate the IC50 value concentration of 35.29μg/ml; ethyl acetate extraction from Reineckia earuea （Andr.） Kunth （12.5, 25.0 and 50.0 g/ml） treatment could significantly inhibit Caski cell proliferation, promote apoptosis （ P 〈 0.05 or P 〈 0.01 ）, when the drug concentration of 50.0 Ixg/ml, time of 48 h, the inhibition rate might up to 98.9% ; up-regulate the mRNA expression level of Bax, downregulate expression levels of COX-2, BC1-2 and BC1-2/Bax ratio compared with the negative control group（ P 〈 0.05 ＆ P 〈 0.01, respectively）. Conclusion： Ethyl acetate extraction from Reineckia earnea （Andr.） Kunth could effectively inhibit proliferation and induce apoptosis in cervix cancer Caski ceils. The mechanism may be related to up-regulating the Bax mRNA expression, down-regulating the COX-2, BC1-2 mRNA expressions, decreasing BCl-2/Bax ratio.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2013年第6期45-50,共6页
Pharmacology and Clinics of Chinese Materia Medica
基金
国家自然科学基金资助项目"民族民间药用植物吉祥草中基于线粒体途径诱导CASKI细胞凋亡活性皂苷的成分解析及其抗肿瘤作用机制研究"
项目号:21272136
关键词
吉祥草乙酸乙酯部位
宫颈癌
CASKI细胞
凋亡
COX-2
ethyl acetate extract from Reineckia carnea （ Andr. ） Kunth （吉祥草乙酸乙酯提取物）
cervix cancer
Caski cells
apopto-sis
COX-2
