摘要
目的：观察养精胶囊对老年雄性大鼠精囊超微结构的影响，探讨其促进精囊分泌功能的作用机制。方法：老年SD雄性大鼠50只，18—20月龄。随机分为空白对照组、十一酸睾酮组（4mg／kg）、养精胶囊高（1．26g/kg）、中（0．63g／kg）、低（0．315g／kg）剂量组共5组，每组10只。分别灌胃30d后，摘取精囊，将精囊液挤人试管称重，测定各组精囊液果糖浓度，双侧精囊分别放人甲醛固定液和戊二醛中行组织学观察。结果：大鼠精囊腺脏器系数（g/g×10^6）、精囊液重量（g）及果糖浓度（mg／ml）分别为：空白对照组1164．5±212．6、0．83±0．30、4．35±0．31；十一酸睾酮组1510．5±313．1、0．82±0．25、5．35±0．71；养精胶囊高剂量组1484．3±262．7、1．14±0．18、5．30±0．45；养精胶囊中剂量组1396．6±268．9、0．83±0．24、4．71±0．41；养精胶囊低剂量组1475．0±305．2、0．74±0．28、4．50±0．23。养精胶囊高剂量组能显著促进精囊液的分泌（P〈0．05），养精胶囊中、低剂量组有改善趋势。苏木素伊红染色后镜下观察，养精胶囊组相对于空白对照组，上皮细胞增生更为活跃，细胞层次增多，细胞胞质丰富透明，内含有较多分泌颗粒、脂肪滴及脂褐素，腺腔腔缘模糊，腔内嗜伊红分泌物，其中结构改变以高剂量组最为显著。养精胶囊组分泌颗粒的面数密度与体密度与空白组比较具有统计学差异（P〈0．05）。结论：养精胶囊可以通过改善精囊腺主细胞的分泌功能，进而促进精囊腺的分泌。
Objective ： To observe the effects of Yangjing Capsule （YJC） on the ultrastructure of seminal vesicles in aged male rats, and explore its mechanism of improving the secretion of seminal vesicles. Methods： Fifty male SD rats aged 18 -20 months were randomly and equally divided into a control group, a testosterone undecanoate group, and a high-dose, a medium-dose and a low-dose YJC group, all fed intragastrically for 30 days. Then the seminal vesicles of the rats were removed and the seminal fluid squeezed into the test tube to be weighed and measured for the concentration of seminal vesicle fluid fructose, and the bilateral seminal vesicles were placed in formaldehyde and glutaraldehyde fixatives for histological observation. Results： The seminal vesicle gland viscera coefficient, seminal vesicle fluid weight and fructose concentration of the rats were [（ 1 164.5 ± 212.6） g/g× 106, （0.83 ± 0.30） g and （4.35 ±0. 31 ） mg/ml in the control group, （ 1 510.5 ± 313.1 ） g/g ×106, （0.82 ± 0. 25 ） g and （ 5.35 ± 0. 71 ） mg/ml in the testosterone undecanoate group, （ 1 484.3 ± 262.7） g/g × 106, （ 1. 14 ± 0. 18 ） g and （5.30 ± 0.45 ） mg/ml in the high-dose YJC group, （1 396.6 ±268.9） g/g × 106, （0.83±0.24） g and （4.71 ±0.41） mg/ml in the medium-dose YJC group, and （1 475.0 ± 305.2） g/g ×106, （0.74 ± 0.28 ） g and （4.50±0.23 ） mg/ml in the low-dose YJC group. Compared with the control, high-dose YJC significantly improved seminal vesicle secretion （ P 〈 0.05 ）, while medium- and low-dose only achieved a trend of improvement. After HE staining, the YJC groups showed more active epithelial hyperplasia, increased cellular layers, rich and transparent cytoplasm with abundant secretory granules, fat droplets and lipofuscin, blurred glandular cavity edge, and eosinophilic intraluminal secretions, as compared with the control group. The structural change was most signifieant in the high-dose group. Statistically significant differences wer
出处
《中华男科学杂志》
CAS
CSCD
2014年第1期68-72,共5页
National Journal of Andrology
基金
国家自然科学基金(81273760/H2709)
国家"973"国家重点基础研究发展计划课题(2010CB530401)~~
关键词
养精胶囊
精囊
超微结构
分泌颗粒
Yangjing Capsule
seminal vesicle
uhrastrueture
secretory granule
