摘要
目的:探讨血链素的抑菌动力学特点;2)检测3种念珠菌菌体细胞内无机磷和大分子物质泄漏情况;3)观察血链素作用3种念珠菌后,菌体细胞超微结构的变化。方法:采用液体培养抑菌法观察血链素分别对C.a、C.t及C.g的抑菌动力学特点;分别检测血链素对C.a、C.t及C.g菌体细胞内磷外泄量及紫外吸收物质渗透率;利用扫描电镜(SEM)和透射电镜(TEM)对血链素作用后的C.a、C.t及C.g菌体细胞超微结构进行观察。结果:血链素对C.a、C.t、C.g两两之间的抑菌动力学特点存在相似性,且血链素对3念珠菌的抑制作用均在24h达高峰。经血链素作用的C.a、C.t及C.g菌体细胞内无机磷及大分子物质发生泄漏。通过SEM观察:血链素对C.a、C.t及C.g作用24h时菌体细胞表面出现凹陷、缺损,菌体呈现不规则形态。通过TEM观察:血链素对C.a作用24h时,菌体细胞皱缩,细胞壁及细胞膜结构分界不清,细胞核消失,胞质不均匀;血链素对C.t作用24h时,菌体出现质壁分离,细胞质固缩,细胞壁及细胞膜连续性破坏或断裂,细胞核溶解;血链素对C.g作用24h时,菌体细胞膜及细胞壁呈现凹坑状缺损,胞核消失。结论:血链素对C.a、C.t及C.g具有相似的抑菌动力学特点。血链素可导致念珠菌菌体细胞膜通透性发生改变。血链素可导致念珠菌菌体细胞的超微结构发生改变。
Objective.. To investigate the characteristics of bacteriostatic dynamics, test the phosphorous leakage and Macromolecular Substances permeability of Candida cells and observe the ultrastructure of three Candida. Meth- ods.. We tested streptococcus sanguis bacteriocin on phosphorous leakage and permeability of Candida. We observed morphological transformation by scanning electron microscopy and transmission electron microscopy in the process of cultivation. Results~ There was similar bacteriostatic dynamics characteristic of Streptococcus sanguis bacteriocin on different Candida and inhibit peak in 24h. Compared with control group, Streptococcus sanguis bacteriocin on candida intracellular inorganic phosphorus and macromolecular Substances reveal significantly. SEM showed that discoid depression and irregular forms were appeared on surface of Candida cell after inhibition for 24h. TEM showed that Candida cells shrinked, nucleus disappeared cytoplasm was inhomogeneous and unclear composition of cell membrane and cell wall were appeared after Streptococcus sanguis bacteriocin on C. a, Cell wall and cell mem- brane of Candida continuity damage or fracture were appeared after Streptococcus sanguis bacteriocin on C. t. discoid depression on cell wall cell membrane of Candida and nucleus disappear were appeared after Streptococcus sanguis bacteriocin on C. g. Conclusion There is similar bacteriostatic dynamics characteristic of Streptococcus sanguis bac- teriocin on different Candida. Streptococcus sanguis bacteriocin on cell membrane permeability and ultrastructure of candida could change.
出处
《口腔医学研究》
CAS
CSCD
2014年第1期21-26,共6页
Journal of Oral Science Research
基金
黑龙江省自然科学基金(编号:D2007-03)
关键词
血链球菌细菌素
念珠菌
抑菌动力学
细胞膜通透性
超微结构
Streptococcus sanguis bacteriocin Candida Bacteriostatic dynamics Membrane permeability UI-trastructure
