摘要
目的在前期采用microPET观察到超早期针刺对脑缺血葡萄糖代谢改善的基础上,从能量代谢角度进一步探讨针刺抗脑缺血损伤的作用机理。方法采用微创开颅电凝法制作大鼠急性脑缺血模型,随机数字表法分为4组:正常组、模型组、穴位组、非穴位组,每组10只。穴位针刺取百会、水沟针刺(频率120次/min,捻转泻法1 min,每次5 min,留针30 min)。非穴位针刺分别在百会、水沟左侧旁开5 mm处进针,避开穴位,手法同穴位针刺。采用免疫组织化学法检测缺血侧皮质区域GLUT1、GLUT3的表达。结果造模成功后,缺血侧皮质区域GLUT1、GLUT3含量在大鼠脑缺血后明显下降,与正常组比较,有极显著性差异(P<0.01);穴位组大鼠GLUT1、GLUT3含量较模型组及非穴位组比较明显升高(P<0.05);而非穴位组与模型组水平接近(P>0.05)。结论穴位针刺可以有效调节脑缺血大鼠缺血侧皮质区域GLUT1、GLUT3的表达,改善能量代谢,发挥抗脑缺血损伤的作用,进一步为临床针刺抗脑缺血提供实验依据。
Objective： To study on the mechanism of anti cerebral ischemia acupuncture from the early micro- PET observation and the very early stage of cerebral ischemia acupuncture which improved glucose metabolism. Methods： The model of acute cerebral ischemia in rats made by minimally invasive craniotomy electrocoagula- tionthe were randomly divided into 4 groups such as the normal group,the model group,the acupuncture group, the non-acupoint group with 10 rats in each group. Acupoint acupuncture involved Baihui （DU20） and Shuigou （DU26） acupuncture （frequency at 120/min,twirling reinforcing-reducing for lmin and retaining the needle for 30min）. Non-acupoint acupuncture needle were Baihui （DU20） and Shuigou （DU26） respectively in its left side about 5 mm,avoiding the acupoints. The needle way was the same with acupoint acupuncture. Expressions in- cluding GLUT1 and GLUT3 were detected by immunohistochemistry in ischemic cortex. Results： After the suc- cess of modeling,the count of ischemic cortex area GLUT1 and GLUT3 were decreased obviously in rats after cerebral ischemia, compared with the normal group ,there is a significant difference between 2 groups （P〈 0.01 ）. The content of GLUT1 and GLUT3 in acupoint group rats compared with model group and non-acupoint group were increased obviously （P〈 0.05）. But non-acupoint group compared with model group, there was no significant difference in the above index （P 〉 0.05）. Conclusion： Acupuncture can improve the ischemic rat cerebral ischemia cortex area energy metabolism, act against cerebral ischemia injury, and further for clinical acupuncture in treating cerebral ischemia provide experimental basis.
出处
《中国中医急症》
2014年第1期51-53,共3页
Journal of Emergency in Traditional Chinese Medicine
基金
湖北中医药大学科研课题(201014)
关键词
针刺
脑缺血
能量代谢
百会穴
水沟穴
Acupuncture
Cerebral ischemia
Energy metabolism
Baihui （DU20）
Shuigou （DU26）
